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Immunofluorescent analysis of SAP130 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SAP130 polyclonal antibody (Product # PA5-32007) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 273 of Human SAP130|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32007 targets SAP130 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-32007 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 273 of Human SAP130.
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
130 kDa Sin3-associated polypeptide; 130 kDa subunit; histone deacetylase complex subunit SAP130; mSin3A-associated protein 130; pre-mRNA splicing factor SF3b; pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; SAP130; SF3b130; Sin3-associated polypeptide p130; Sin3A associated protein 130kDa; Sin3A associated protein, 130kDa; Sin3A-associated protein, 130kDa; spliceosome-associated protein 130; splicing factor 3B subunit 3
2610304F09Rik; 6720406D06; SAP130