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SAP97 Polyclonal Antibody
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FIGURE: 1 / 2
SAP97 Antibody (PA1-044) in WB
Western blot analysis was performed on membrane extracts (30 µglysate) of HeLa (Lane 1), and MCF-7 (Lane 2). The blots were probed with Rabbit Anti-SAP97 Polyclonal Antibody (Product # PA1-044, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to SAP97 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-044 detects synapse-associated protein 97 (SAP97) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.
PA1-044 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain homogenate.
The PA1-044 immunogen is a synthetic peptide corresponding to residues E(16) E Y R S K L S Q T E D R Q L R S S(33) of human SAP97. This sequence is completely conserved in rat and mouse. PA1-044 immunizing peptide (Cat. # PEP-022) is available for use in neutralization and control experiments.
Target Information
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; dJ1061C18.1.1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); presynaptic protein SAP97; synapse-associated protein 97; unnamed protein product
Gene Aliases: DLGH1; hdlg; SAP-97; SAP97
UniProt ID: (Human) Q12959, (Rat) Q62696
Entrez Gene ID: (Human) 1739, (Rat) 25252
guanylate kinase activity
phosphoprotein phosphatase activity
protein binding
cytoskeletal protein binding
potassium channel regulator activity
kinase binding
protein kinase binding
phosphatase binding
ionotropic glutamate receptor binding
ion channel binding
cadherin binding
binding, bridging
L27 domain binding
structural constituent of postsynaptic density
kinesin binding
enzyme binding
PDZ domain binding
negative regulation of transcription from RNA polymerase II promoter
endothelial cell proliferation
actin filament organization
establishment or maintenance of cell polarity
chemical synaptic transmission
nervous system development
regulation of cell shape
cortical actin cytoskeleton organization
astral microtubule organization
protein complex localization
regulation of protein localization
protein localization to synapse
regulation of membrane potential
receptor clustering
regulation of potassium ion transport
positive regulation of potassium ion transport
cortical microtubule organization
establishment or maintenance of epithelial cell apical/basal polarity
GMP metabolic process
GDP metabolic process
establishment of centrosome localization
negative regulation of ERK1 and ERK2 cascade
bicellular tight junction assembly
protein localization to membrane
protein localization to plasma membrane
receptor localization to synapse
cell-cell adhesion
regulation of ventricular cardiac muscle cell action potential
membrane repolarization during ventricular cardiac muscle cell action potential
regulation of postsynaptic membrane neurotransmitter receptor levels
maintenance of postsynaptic density structure
neurotransmitter receptor localization to postsynaptic specialization membrane
regulation of NIK/NF-kappaB signaling
regulation of sodium ion transmembrane transport
regulation of protein localization to synapse
positive regulation of protein localization to plasma membrane
regulation of potassium ion import
negative regulation of p38MAPK cascade
regulation of potassium ion export across plasma membrane
protein localization to cell periphery
negative regulation of G1/S transition of mitotic cell cycle
ureteric bud development
branching involved in ureteric bud morphogenesis
immunological synapse formation
lens development in camera-type eye
cell adhesion
protein localization
positive regulation of cell proliferation
positive regulation of gene expression
epithelial structure maintenance
cerebral cortex development
peristalsis
positive regulation of actin filament polymerization
membrane raft organization
regulation of myelination
activation of protein kinase activity
T cell activation
negative regulation of T cell proliferation
amyloid precursor protein metabolic process
reproductive structure development
embryonic skeletal system morphogenesis
tissue morphogenesis
smooth muscle tissue development
negative regulation of epithelial cell proliferation
negative regulation of protein kinase B signaling
hard palate development
cellular response to brain-derived neurotrophic factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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