PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain extract. Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining.
The PA1-741 immunizing peptide corresponds to amino acids 115-133 from rat SAP97. This sequence is 84% and 74% conserved in mouse and human SAP97, respectively.
PA1-741 can be used with blocking peptide PEP-055.
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
Protein Aliases: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); E-dlg/SAP97; Embryo-dlg/synapse-associated protein 97; hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97
Gene Aliases: B130052P05Rik; dJ1061C18.1.1; DLG1; DLGH1; E-dlg/SAP97; hdlg; mKIAA4187; SAP-97; SAP97
Molecular Function: transmembrane receptor regulatory/adaptor protein