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SARA Polyclonal Antibody

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Antibody Testing Data (8)

SARA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

SARA Antibody (22033-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 40x lens).

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SARA Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 22033-1-AP) SARA antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

SARA Antibody in Immunoprecipitation (IP) — IP result of anti-SARA (IP:22033-1-AP, 5ug; Detection:22033-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

Product Details

22033-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SARA Fusion Protein Ag16929 (1075-1425 aa encoded by BC032680)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MLRLGAEYR LYPCPLFSVR FRKPLFGETG HTIMNLLADF RNYQYTLPVV QGLVVDMEVR KTSIKIPSNR YNEMMKAMNK SNEHVLAGGA CFNEKADSHL VCVQNDDGNY QTQAISIHNQ PRKVTGASFF VFSGALKSSS GYLAKSSIVE DGVMVQITAE NMDSLRQALR EMKDFTITCG KADAEEPQEH IHIQWVDDDK NVSKGVVSPI DGKSMETITN VKIFHGSEYK ANGKVIRWTE VFFLENDDQH NCLSDPADHS RLTEHVAKAF CLALCPHLKL LKEDGMTKLG LRVTLDSDQV GYQAGSNGQP LPSQYMNDLD SALVPVIHGG ACQLSEGPVV MELIFYILEN IV (1075-1425 aa encoded by B C032680)

Target Information

Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dysfunction of Caveolae-Mediated Endocytic TβRI Degradation Results in Hypersensitivity of TGF-β/Smad Signaling in Osteogenesis Imperfecta.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Shi C,Sun B,Wu H,Zhang R,Wu L,Guo L,Li C,Xi Y,Yuan W,Zhang Y,Xu G

Sun Jan 01 00:00:00 EST 2023

Dysfunction of Caveolae-Mediated Endocytic TβRI Degradation Results in Hypersensitivity of TGF-β/Smad Signaling in Osteogenesis Imperfecta.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Shi C,Sun B,Wu H,Zhang R,Wu L,Guo L,Li C,Xi Y,Yuan W,Zhang Y,Xu G

Sun Jan 01 00:00:00 EST 2023

Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis.

Science advances

Lu ZZ,Sun C,Zhang X,Peng Y,Wang Y,Zeng Y,Zhu N,Yuan Y,Zeng MS

Wed May 24 00:00:00 EDT 2023

Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis.

Science advances

Lu ZZ,Sun C,Zhang X,Peng Y,Wang Y,Zeng Y,Zhu N,Yuan Y,Zeng MS

Wed May 24 00:00:00 EDT 2023

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Structure (London, England : 1993)

Gahloth D,Levy C,Walker L,Wunderley L,Mould AP,Taylor S,Woodman P,Tabernero L

Wed Jul 05 00:00:00 EDT 2017

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Structure (London, England : 1993)

Gahloth D,Levy C,Walker L,Wunderley L,Mould AP,Taylor S,Woodman P,Tabernero L

Wed Jul 05 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: hSARA; MAD, mothers against decapentaplegic homolog interacting protein, receptor activation anchor; MADH-interacting protein; Mothers against decapentaplegic homolog-interacting protein; Novel serine protease; NSP; protein phosphatase 1, regulatory subunit 173; Receptor activation anchor; Smad anchor for receptor activation; Zinc finger FYVE domain-containing protein 9; zinc finger, FYVE domain containing 9

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Gene Aliases: E030027L17; MADHIP; NSP; PPP1R173; SARA; SMADIP; ZFYVE9

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UniProt ID: (Human) O95405

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Entrez Gene ID: (Human) 9372, (Rat) 313477, (Mouse) 230597

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Function(s)

transforming growth factor beta receptor signaling pathway SMAD protein complex assembly SMAD protein import into nucleus biological_process proteolysis endocytosis regulation of transforming growth factor beta receptor signaling pathway

Process(es)

protein binding 1-phosphatidylinositol binding serine-type peptidase activity metal ion binding molecular_function SMAD binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SARA Polyclonal Antibody

SARA Antibody (22033-1-AP) in IHC (P)

Product Details

22033-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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