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SART1 Antibody (PA5-97120) in IHC (P)
Immunohistochemistry analysis of SART1 in paraffin-embedded human esophageal. Samples were incubated with SART1 Polyclonal antibody (Product # PA5-97120) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-97120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_0051371)
Conjugate
Form
Liquid
Concentration
0.79 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808922
Product Specific Information
Immunogen sequence: MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS ARDLQ; Positive Samples: HeLa, Jurkat, HepG2, Mouse liver, Mouse brain, Mouse testis, Rat brain; Cellular Location: Nucleus
Target Information
This gene encodes two proteins, the SART1protein expressed in the nucleus of the majority of proliferating cells, and the SART1protein expressed in the cytosol of epithelial cancers. The SART1protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSART-1; hSnu66; Hypoxia-associated factor; IgE autoantigen; MGC2038; mSART-1; rSART-1; SART-1; SART1(259) protein; SART1(800) protein; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNU66 homolog; squamous cell carcinoma antigen recognised by T cells; squamous cell carcinoma antigen recognized by T cells 1; Squamous cell carcinoma antigen recognized by T-cells 1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1
Gene Aliases: Ara1; Haf; HOMS1; SART1; SART1259; SNRNP110; Snu66; U5-110K
UniProt ID: (Human) O43290, (Mouse) Q9Z315, (Rat) Q5XIW8
Entrez Gene ID: (Human) 9092, (Mouse) 20227, (Rat) 29678
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