Invitrogen

SASH3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (2) SASH3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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SASH3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of SASH3 in human tonsil using a SASH3 Polyclonal Antibody (Product # PA5-51511) shows cytoplasmic positivity in non-germinal and germinal center cells.

SASH3 Antibody in Western Blot (WB) — Western blot analysis of SASH3 in control (vector only transfected HEK293T lysate) and SASH3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SASH3 Polyclonal Antibody (Product # PA5-51511).

SASH3 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of SASH3 demonstrates antibody specificity. Immunohistochemical analysis of SASH3 using anti-SASH3 Polyclonal Antibody (Product # PA5-51511), shows significant staining of SASH3 in human tonsil and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of SASH3 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-51511

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human SASH3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.10 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2646960

Product Specific Information

Immunogen sequence: PKTLHELLER IGLEEHTSTL LLNGYQTLED FKELRETHLN ELNIMDPQHR AKLLTAAELL LDYDTGSEEA EEGAESSQEP VAHTVSEPKV DIPRDSGCFE GSESGRDDAE LAGTEEQLQG LSL

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 97%.

Target Information

The protein encoded by this gene contains a Src homology-3 (SH3) domain and a sterile alpha motif (SAM), both of which are found in proteins involved in cell signaling. This protein may function as a signaling adapter protein in lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.