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SCAMP1 Antibody (PA1-739) in WB
Western blot analysis was performed on membrane extracts (30 µglysate) of U-87 MG (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), SK-N-AS (Lane 4), T98G (Lane5), IMR-32 (Lane 6), and tissue extract (30 µglysate) of rat brain (Lane 7). The blots were probed with Rabbit Anti-SCAMP1 Polyclonal Antibody (Product # PA1-739, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to SCAMP1 was obs... View More
Product Details
PA1-739
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: M(1) S D F D S N P F A D P D L N N P(17) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2184624
Product Specific Information
PA1-739 detects secretory carrier membrane protein 1 (SCAMP 1) from human, mouse and rat samples.
PA1-739 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 in rat and mouse brain tissues as well as HeLa cell lysates.
PA1-739 immunizing peptide corresponds to amino acid residues 1-17 from human SCAMP 1. This sequence is completely conserved between human, rat, and porcine SCAMP 1. PA1-739 immunizing peptide (Cat. # PEP-105) is available for use in neutralization and control experiments.
Target Information
Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These ubiquitously expressed proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. Of the three known SCAMPs, SCAMP 1 is the most abundant. SCAMP 1 has been shown to be required for the full execution of stable exocytosis in mast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: SCAMP 37; Secretory carrier membrane protein 1; Secretory carrier-associated membrane protein 1
Gene Aliases: 4930505M11Rik; AI415563; AW122395; SCAMP; SCAMP1; SCAMP37
UniProt ID: (Human) O15126, (Rat) P56603, (Mouse) Q8K021
Entrez Gene ID: (Human) 9522, (Rat) 29521, (Mouse) 107767
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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