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Invitrogen
SCAMP2 Polyclonal Antibody
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FIGURE: 1 / 4
SCAMP2 Antibody (PA1-734) in WB
Western blot analysis was performed on membrane extracts (30 µglysate) of U-87 MG (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), SK-N-AS (Lane 4), and T98G (Lane 5). The blots were probed with Rabbit Anti-SCAMP2 Polyclonal Antibody (Product # PA1-734, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to SCAMP1 was observed across the cell lines and tissues tested. Known quantity of pr... View More
Product Details
PA1-734
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: M(1) S A F D T N P F A D P V D V N P(17) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-734 detects secretory carrier membrane protein 2 (SCAMP 2) from mouse testes and AtT20 cell extract as well as Chinese hamster ovary (CHO) cell extract.
PA1-734 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing SCAMP 2 from CHO cell extract.
PA1-734 immunizing peptide corresponds to amino acid residues 1-17 from human SCAMP 2. This sequence is completely conserved between human and mouse. PA1-734 immunizing peptide (Cat. # PEP-151) is available for use in neutralization and control experiments.
Target Information
Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: scamp 2; SCAMP2; Secretory carrier membrane protein 2; Secretory carrier-associated membrane protein 2; similar to Rattus norvegicus SCAMP37, PIR Accession Number S37395; testis secretory sperm-binding protein Li 219p; unnamed protein product
Gene Aliases: AI875466; I79_023583; Sc2; SCAMP2
UniProt ID: (Human) O15127, (Mouse) Q9ERN0
Entrez Gene ID: (Human) 10066, (Chinese hamster) 100756209, (Mouse) 24044
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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