|Tested species reactivity||Human, Pig|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCD.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody is predicted to react with mouse based on sequence homology.
Stearoyl-CoA desaturase is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5; Wang et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Discovery of tumor-specific irreversible inhibitors of stearoyl CoA desaturase.
PA5-19682 was used in immunoprecipitation and western blot to characterize inhibitors of stearoyl CoA desaturase as cancer therapeutics
|Theodoropoulos PC,Gonzales SS,Winterton SE,Rodriguez-Navas C,McKnight JS,Morlock LK,Hanson JM,Cross B,Owen AE,Duan Y,Moreno JR,Lemoff A,Mirzaei H,Posner BA,Williams NS,Ready JM,Nijhawan D||Nature chemical biology (12:218)||2016|