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SCG3 Antibody (PA5-121140) in WB
Western blot analysis of SCG3 in extracts of various cell lines. Samples were incubated with SCG3 Polyclonal antibody (Product # PA5-121140) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-121140
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 199-468 of human SCG3 (NP_037375.2).
Conjugate
Form
Liquid
Concentration
1.09 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914712
Product Specific Information
Positive test controls include: 22Rv1, HepG2, Mouse brain, Mouse lung, Mouse heart, Rat brain, Rat lung, Rat heart. The target is usually found in the following locations: Cytoplasmic vesicle, Peripheral membrane protein, Secreted, secretory vesicle lumen, secretory vesicle membrane.
Immunogen sequence: KLISKEANNY EEDPNKPTSW TENQAGKIPE KVTPMAAIQD GLAKGENDET VSNTLTLTNG LERRTKTYSE DNFEELQYFP NFYALLKSID SEKEAKEKET LITIMKTLID FVKMMVKYGT ISPEEGVSYL ENLDEMIALQ TKNKLEKNAT DNISKLFPAP SEKSHEETDS TKEEAAKMEK EYGSLKDSTK DDNSNPGGKT DEPKGKTEAY LEAIRKNIEW LKKHDKKGNK EDYDLSKMRD FINKQADAYV EKGILDKEEA EAIKRIYSSL
Target Information
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1B1075; Secretogranin III; Secretogranin-3; SgIII
Gene Aliases: 1B1075; AI385542; Chgd; SCG3; SGIII; UNQ2502/PRO5990
UniProt ID: (Human) Q8WXD2, (Rat) P47868, (Mouse) P47867
Entrez Gene ID: (Human) 29106, (Rat) 116635, (Mouse) 20255
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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