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Invitrogen
SCNN1G Polyclonal Antibody
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Product Details
PA1-922
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Amphibian,
C. elegans,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues L(630) R L E R A F S N Q L T D T Q M L D E L(649) of rat gamma-ENaC.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-922 detects recombinant rat gamma-epithelial sodium channel (gamma-ENaC).
PA1-922 has been successfully used in immunoprecipitation procedures. By immunoprecipitation this antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma-ENaC protein.
This product fails to detect gamma-ENaC protein in Western blot procedures.
PA1-922 immunizing peptide corresponds to amino acid residues 630-649 from human gamma-ENaC. This sequence is 76%, 71%, and 66% conserved in rat, mouse, and rabbit gamma-ENaC, respectively. PA1-922 immunizing peptide (Cat. # PEP-090) is available for use in neutralization and control experiments.
Target Information
Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: amiloride sensitive sodium channel gamma1 subunit; Amiloride-sensitive sodium channel subunit gamma; Epithelial Na(+) channel subunit gamma; epithelial sodium channel gamma subunit; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; SCNEG; SCNN 1G; Sodium channel nonvoltage-gated 1 gamma (epithelial); Sodium channel, nonvoltage-gated 1, gamma (epithelial); sodium channel, nonvoltage-gated, type I, gamma
Gene Aliases: ENaC; Scnn1g
UniProt ID: (Rat) P37091
Entrez Gene ID: (Rat) 24768
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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