PA1-922 detects recombinant rat gamma-epithelial sodium channel (gamma-ENaC).
PA1-922 has been successfully used in immunoprecipitation procedures. By immunoprecipitation this antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma-ENaC protein.
This product fails to detect gamma-ENaC protein in Western blot procedures.
PA1-922 immunizing peptide corresponds to amino acid residues 630-649 from human gamma-ENaC. This sequence is 76%, 71%, and 66% conserved in rat, mouse, and rabbit gamma-ENaC, respectively. PA1-922 immunizing peptide (Cat. # PEP-090) is available for use in neutralization and control experiments.
Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: amiloride sensitive sodium channel gamma1 subunit; Amiloride-sensitive sodium channel subunit gamma; Epithelial Na(+) channel subunit gamma; epithelial sodium channel gamma subunit; Gamma ENaC; Gamma NaCH; Gamma-ENaC; Gamma-NaCH; Nonvoltage-gated sodium channel 1 subunit gamma; PHA 1; SCNEG; SCNN 1G; Sodium channel nonvoltage-gated 1 gamma (epithelial); Sodium channel, nonvoltage-gated 1, gamma (epithelial); sodium channel, nonvoltage-gated, type I, gamma
Gene Aliases: ENaC; Scnn1g
UniProt ID: (Rat) P37091
Entrez Gene ID: (Rat) 24768