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Invitrogen
SCO2 Recombinant Rabbit Monoclonal Antibody (PSH09-97)
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FIGURE: 1 / 6
SCO2 Antibody (MA570105) in ICC/IF
Immunocytochemistry analysis of SCO2 in MCF7 cells. Samples were incubated with SCO2 monoclonal antibody (Product # MA570105) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was u... View More
Product Details
MA570105
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH09-97
Immunogen
Recombinant protein within human SCO2 aa 61-266.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: MCF7 cell lysate, HL-60 cell lysate, SK-OV-3 cell lysate, HepG2 cell lysate, U-937 cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Mitochondrion inner membrane.
Target Information
Synthesis of cytochrome c oxidase 2 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, the drop in SCO2 expression leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in the SCO2 protein are also associated with fatal infantile cardioencephalomyopathy and COX deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein SCO2 homolog, mitochondrial; SCO cytochrome oxidase deficient homolog 2
Gene Aliases: CEMCOX1; MYP6; SCO1L; SCO2
UniProt ID: (Human) O43819, (Mouse) Q8VCL2
Entrez Gene ID: (Human) 9997, (Mouse) 100126824
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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