Invitrogen

SCXA Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (7) SCXA antibodies

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Protocols

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SCXA Antibody in Western Blot (WB) — Western blot analysis using a SCXA polyclonal antibody (Product # PA5-23943) in mouse cerebellum tissue lysates (35 µg per lane).

SCXA Antibody — Figure 3 cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. ( a ) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 ug/mL) in the presence or absence of TGFbeta1 (10 ng/mL). beta-tubulin, as loading control, was from the same samples. ( b,d ) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFbeta1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. ( c ) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. ( e ) Relative mRNA expression of ID3 in fibroblasts treated with 12 ug/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. ( f ) Relative mRNA expression of ID3 in control (F ctrl ) and ID3-knockdown (F [?]ID3 ) fibroblasts treated {TM}

SCXA Antibody in Western Blot, ChIP assay (WB, ChIP) — Figure 3 cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. ( a ) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 ug/mL) in the presence or absence of TGFbeta1 (10 ng/mL). beta-tubulin, as loading control, was from the same samples. ( b,d ) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFbeta1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. ( c ) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. ( e ) Relative mRNA expression of ID3 in fibroblasts treated with 12 ug/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. ( f ) Relative mRNA expression of ID3 in control (F ctrl ) and ID3-knockdown (F ID3 ) fibroblasts treated fo

Product Details

PA5-23943

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 5 publications 5 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
ChIP assay (ChIP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2541443

Product Specific Information

This antibody is predicted to react with chicken based on sequence homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Copper Does Not Induce Tenogenic Differentiation but Promotes Migration and Increases Lysyl Oxidase Activity in Adipose-Derived Mesenchymal Stromal Cells.

Stem cells international

Milewska M,Burdzińska A,Zielniok K,Siennicka K,Struzik S,Zielenkiewicz P,Pączek L

PA5-23943 was used in Western Blot to verify whether copper supplementation may have a positive effect on the properties of human adipose tissue-derived MSCs (hASCs) which potentially can contribute to improvement of tendon healing.

Wed Apr 13 00:00:00 EDT 2022

The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells.

Stem cells international

Zarychta-Wiśniewska W,Burdzińska A,Zielniok K,Koblowska M,Gala K,Pędzisz P,Iwanicka-Nowicka R,Fogtman A,Aksamit A,Kulesza A,Zołocińska A,Pączek L

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Fri Apr 08 00:00:00 EDT 2022

The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.

International journal of stem cells

Bochon K,Zielniok K,Gawlak M,Zawada K,Zarychta-Wiśniewska W,Siennicka K,Struzik S,Pączek L,Burdzińska A

PA5-23943 was used in Western Blot, Immunocytochemistry to investigate if serum reduction, L-ascorbic acid supplementation or a combination of both factors can induce tenogenic differentiation of human adipose-derived MSCs (ASCs).

Sun Feb 28 00:00:00 EST 2021

The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.

International journal of stem cells

Bochon K,Zielniok K,Gawlak M,Zawada K,Zarychta-Wiśniewska W,Siennicka K,Struzik S,Pączek L,Burdzińska A

Sun Feb 28 00:00:00 EST 2021

The Transcription Factor SCX is a Potential Serum Biomarker of Fibrotic Diseases.

International journal of molecular sciences

Ramírez-Aragón M,Hernández-Sánchez F,Rodríguez-Reyna TS,Buendía-Roldán I,Güitrón-Castillo G,Núñez-Alvarez CA,Hernández-Ramírez DF,Benavides-Suárez SA,Esquinca-González A,Torres-Machorro AL,Mendoza-Milla C

PA5-23943 was used in Western Blotting to propose the bHLH transcription factor scleraxis (SCX) could be a candidate biomarker of fibrotic diseases.

Thu Jul 16 00:00:00 EDT 2020

Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.

Scientific reports

Teo Z,Chan JSK,Chong HC,Sng MK,Choo CC,Phua GZM,Teo DJR,Zhu P,Choong C,Wong MTC,Tan NS

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Mon Jul 24 00:00:00 EDT 2017

Angiopoietin-like 4 induces a β-catenin-mediated upregulation of ID3 in fibroblasts to reduce scar collagen expression.

Scientific reports

Teo Z,Chan JSK,Chong HC,Sng MK,Choo CC,Phua GZM,Teo DJR,Zhu P,Choong C,Wong MTC,Tan NS

Published figure using SCXA polyclonal antibody (Product # PA5-23943) in Western Blot

Mon Jul 24 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; class II bHLH protein scleraxis; scleraxis basic helix-loop-helix transcription factor; scleraxis homolog A; scleraxis homolog B

Gene Aliases: BB114693; BHLHA41; BHLHA48; Scl; SCX; SCXA; SCXB

UniProt ID: (Human) Q7RTU7, (Mouse) Q64124

Entrez Gene ID: (Human) 642658, (Mouse) 20289

Molecular Function: basic helix-loop-helix transcription factor