Immunohistochemistry analysis of SCXA in paraffin-embedded Human breast carcinoma tissue. Samples were incubated with SCXA polyclonal antibody (Product # PA5-23943) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0)... View More

SCXA Antibody in Immunocytochemistry (ICC/IF)

SCXA Antibody in Western Blot, ChIP Assay (WB, ChIP)

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SCXA Antibody — Figure 3 cANGPTL4 regulates COL1A2 and COL3A1 expression in fibroblasts via a bHLH-dependent signaling mechanism. ( a ) Immunoblot analysis of indicated proteins from fibroblasts treated with cANGPTL4 (12 ug/mL) in the presence or absence of TGFbeta1 (10 ng/mL). beta-tubulin, as loading control, was from the same samples. ( b,d ) Chromatin immunoprecipitation (ChIP) of SBE sites in the human COL1A2 gene promoter region and E-box sites in the human COL1A2 and COL3A1 gene promoter regions using phospho-Smad3 antibodies and scleraxis (ScxA) antibodies respectively. The gene sequences spanning the SBE and E-box sites and a random control sequence were analyzed by PCR from immunoprecipitated chromatin from fibroblasts treated with TGFbeta1 in the presence or absence of cANGPTL4 (cANG). Pre-immune serum was used as a control. PCR was performed using chromatin before immunoprecipitation as input. M, 100 bp DNA ladder. ( c ) A schematic diagram showing the relative positions of Enhancer boxes (E-Boxes) and Smad binding elements (SBE) on the human COL1A2 and COL3A1 proximal promoters. Sequences of E-Boxes and SBE are shown. ChIP PCR primers designed specifically to bind scleraxis along this proximal promoter region are indicated. ( e ) Relative mRNA expression of ID3 in fibroblasts treated with 12 ug/mL of cANGPTL4 and lysed for mRNA at the indicated time points post-treatment. ( f ) Relative mRNA expression of ID3 in control (F ctrl ) and ID3-knockdown (F [delta]ID3 ) fibroblasts treated {TM}

Immunogen image

Immunogen Image SCXA Polyclonal Antibody

Product Details

PA5-23943

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2541443

Product Specific Information

This antibody is predicted to react with chicken based on sequence homology.

Target Information

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and bHLH transcription factor binding activity. Contributes to E-box binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of transcription regulator complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; class II bHLH protein scleraxis; scleraxis basic helix-loop-helix transcription factor; scleraxis homolog A; scleraxis homolog B

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Gene Aliases: BB114693; BHLHA41; BHLHA48; Scl; SCX; SCXA; SCXB

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UniProt ID: (Human) Q7RTU7, (Mouse) Q64124

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Entrez Gene ID: (Human) 642658, (Mouse) 20289

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Function(s)

RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding bHLH transcription factor binding sequence-specific DNA binding protein heterodimerization activity E-box binding protein binding protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

mesoderm formation tissue homeostasis endochondral ossification chondrocyte differentiation heart valve morphogenesis heart valve formation transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of gene expression cell differentiation collagen fibril organization BMP signaling pathway positive regulation of collagen biosynthetic process skeletal muscle cell differentiation tendon development tendon cell differentiation tendon formation deltoid tuberosity development negative regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic skeletal system development Sertoli cell differentiation face morphogenesis regulation of cartilage development positive regulation of cartilage development sclerotome development cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus positive regulation of gastrulation regulation of transcription, DNA-templated multicellular organism development cartilage development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SCXA Polyclonal Antibody

SCXA Antibody (PA5-23943) in IHC (P)

Product Details

PA5-23943

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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