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Invitrogen
SDHB Polyclonal Antibody
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FIGURE: 1 / 8
SDHB Antibody (PA5-18407) in ICC/IF
Immunocytochemistry analysis of SDHB using SDHB Polyclonal Antibody (Product # PA5-18407) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (ATYKEKKASV) corresponding to the C-terminus amino acids of SDHB (aa 271-280).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ92337; Ip; iron-sulfur subunit; Iron-sulfur subunit of complex II; Malate dehydrogenase [quinone] iron-sulfur subunit; OTTHUMP00000044624; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur (Ip); succinate-ubiquinone oxidoreductase Ip subunit precursor; unnamed protein product
Gene Aliases: CWS2; IP; MC2DN4; PGL4; PPGL4; SDH; SDH1; SDH2; SDHB; SDHIP
UniProt ID: (Human) P21912
Entrez Gene ID: (Human) 6390
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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