Invitrogen

SDHB Polyclonal Antibody

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SDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SDHB on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with SDHB polyclonal antibody (Product# PA5-79965) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

SDHB Antibody in Western Blot (WB) — Western blot was performed using Anti-SDHB Polyclonal Antibody, (Product # PA5-79965) and a 30 kDa band corresponding to SDHB was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), MCF-7 (Lane 3), HEK-293 (Lane 4), tissue extracts (40 µg lysate) of Mouse Kidney (Lane 5) and Rat Kidney (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with SDHB Polyclonal Antibody (Product # PA5-79965, 1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed around 50 kDa molecular weight.

Product Details

PA5-79965

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Immunocytochemistry (ICC/IF)	2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived human SDHB recombinant protein (Position: A29-V280). View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	-20°C
RRID	AB_2747080

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ92337; Ip; iron-sulfur subunit; Iron-sulfur subunit of complex II; OTTHUMP00000044624; succinate dehydrogenase [ubiquinone] iron-sulfur subunit; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex subunit B; succinate dehydrogenase complex subunit B, iron sulfur (Ip); succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Aliases: 0710008N11Rik; CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHB; SDHIP

UniProt ID: (Human) P21912, (Mouse) Q9CQA3, (Rat) P21913

Entrez Gene ID: (Human) 6390, (Mouse) 67680, (Rat) 298596

Molecular Function: dehydrogenase