|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid synthetic peptide near the center of human SDHD|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2.5 ug/mL|
|Immunofluorescence (IF)||2.5 ug/mL|
|Western Blot (WB)||1 - 2 ug/mL|
|Western Blot (WB)||See 1 publications below|
A suggested positive control is EL4 cell lysate.
PA5-34387 can be used with blocking peptide PEP-1430.
The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.
High fat, high sucrose diet causes cardiac mitochondrial dysfunction due in part to oxidative post-translational modification of mitochondrial complex II.
PA5-34387 was used in western blot to study the correlation between cardiac mitochondrial dysfunction and the high fat, high sucrose diet
|Sverdlov AL,Elezaby A,Behring JB,Bachschmid MM,Luptak I,Tu VH,Siwik DA,Miller EJ,Liesa M,Shirihai OS,Pimentel DR,Cohen RA,Colucci WS||Journal of molecular and cellular cardiology (78:165)||2015|
CBT1; CII-4; cybS; PGL; PGL1; QPs3; SDH4; SDHD; succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial; succinate dehydrogenase complex subunit D; succinate dehydrogenase complex subunit D integral membrane protein; succinate dehydrogenase cytochrome b small subunit mitochondrial; succinate-ubiquinone oxidoreductase cytochrome b small subunit; succinate-ubiquinone reductase membrane anchor subunit
3110001M13Rik; AVLL5809; C78570; CBT1; CII-4; CWS3; cybS; PGL; PGL1; PRO19626; QPs3; SDH4; SDHD