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SEMA3A Polyclonal Antibody
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FIGURE: 1 / 3
SEMA3A Antibody (PA5-67972) in WB
Western blot was performed using Anti-SEMA3A Polyclonal Antibody (Product # PA5-67972) and a 82 kDa band corresponding to SEMA3A was observed across cell lines tested except Hep G2 and Jurkat which are reported to be negative. The expression of protein is also seen in Mouse and Rat Brain tissue when compared to Mouse and Rat Lung tissue which is reported to be low. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), Neuro-2a (Lane 3), Hep G2 (Lane 4) and Jurkat (Lane 5) and tissue extracts of Mouse Lung (Lane 6), Rat L... View More
Product Details
PA5-67972
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from human SEMA3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted to react with Mouse and Rat samples.
Target Information
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: collapsin 1; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Sema III; semaphorin D; Semaphorin III; semaphorin L; Semaphorin-3A; unnamed protein product
Gene Aliases: coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMA3A; SEMAD; SEMAIII; SEMAL; SemD
UniProt ID: (Human) Q14563
Entrez Gene ID: (Human) 10371
neural crest cell migration
neuron migration
nervous system development
axon guidance
motor neuron axon guidance
negative regulation of neuron projection development
facial nerve structural organization
trigeminal nerve structural organization
nerve development
olfactory bulb development
branchiomotor neuron axon guidance
cell differentiation
positive regulation of cell migration
forebrain development
positive regulation of JNK cascade
sympathetic nervous system development
regulation of axon extension involved in axon guidance
axon extension involved in axon guidance
sensory system development
negative chemotaxis
axonogenesis involved in innervation
sympathetic ganglion development
semaphorin-plexin signaling pathway
sympathetic neuron projection extension
sympathetic neuron projection guidance
basal dendrite arborization
neural crest cell migration involved in autonomic nervous system development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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