|Tested species reactivity||Bovine, Dog, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 100-200 of the human SEMA 3B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: antigen standard for SEMA3B (transient overexpression lysate), mouse Cerebellum protein, mouse E16 cerebellum lysate.
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. SEMA 3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.