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SEMG2 Antibody (PA5-88785) in WB
Western blot analysis of SEMG2 in extracts of HeLa. Samples were incubated with SEMG2 Polyclonal antibody (Product # PA5-88785) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 383-582 of human SEMG2 (NP_0029991)
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805129
Product Specific Information
Immunogen sequence: HGKSQNQVRI PSQAQEYGHK ENKISYQSSS TEERRLNSGE KDVQKGVSKG SISIQTEEKI HGKSQNQVTI PSQDQEHGHK ENKMSYQSSS TEERRLNYGG KSTQKDVSQS SISFQIEKLV EGKSQIQTPN PNQDQWSGQN AKGKSGQSAD SKQDLLSHEQ KGRYKQESSE SHNIVITEHE VAQDDHLTQQ YNEDRNPIST; Positive Samples: 293T, HeLa, Mouse testis
Target Information
The secreted protein encoded by this gene is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Semenogelin 2; Semenogelin II; Semenogelin-2; seminal vesicle protein 3; seminal vesicle secretion 3; seminal vesicle secretion 3 alpha; seminal vesicle secretion 3A; Seminal vesicle secretory protein 3A; Seminal vesicle secretory protein III; SGII; SVS III
Gene Aliases: 9530026M05Rik; BB121242; SEMG2; SGII; Svp-3; Svp3; Svs3; Svs3a
UniProt ID: (Human) Q53ZU2, (Mouse) F2Z472
Entrez Gene ID: (Human) 6407, (Mouse) 64335
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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