Invitrogen

SERPINA12 Polyclonal Antibody

3 Published Figures

View all (11) SERPINA12 antibodies

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SERPINA12 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SERPINA12 in paraffin-embedded colon carcinoma using a SERPINA12 polyclonal antibody (Product # PA5-30989) at a dilution of 1:250.

SERPINA12 Antibody in Western Blot (WB) — Western blot analysis of SERPINA12 using 30 µg of Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SERPINA12 polyclonal antibody (Product # PA5-30989) at a dilution of 1:5000.

SERPINA12 Antibody in Immunocytochemistry (ICC/IF) — Figure 1 Vaspin mRNA and protein expression before and after oocyte in vitro maturation, as well as its immunolocalisation in cumulus-oocyte complexes (COCs). COCs (50/group/experiment) were selected after morphological examination of material collected from the ovaries taken from 100 pigs (15 follicles/ovary). COCs were collected before (0 h) and after (44 h) in vitro maturation and oocytes were denuded by gently pipetting in hyaluronidase, then real-time PCR and Western blot analysis were performed to determine mRNA ( A ) and protein ( B ) level of vaspin in oocytes and cumulus cells. Additionally, immunolocalisation of vaspin was analysed by immunofluorescence in COCs ( C ). Gene expression level was normalised to the geometric mean of actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and protein to actin. Vaspin immunostaining shown by arrows. Experiments were performed independently and repeated three times ( n = 3 ). The data are plotted as the mean +- standard error of the mean (SEM) of three independent experiments. Significance between groups before and after maturation is indicated by *** p < 0.001; Cumulus cells (Cc), oocyte (Oo), Immunoglobulin G (IgG).

SERPINA12 Antibody in Immunohistochemistry (IHC) — Figure 1 Qualitative ( A - H , A' - H' ) and quantitative ( I ) analyses of immunohistochemical staining of chemerin ( A , A' ), CCRL2 ( B , B' ), CMKLR1 ( C , C' ), GPR1 ( D , D' ), apelin ( E , E' ), APLNR ( F , F' ), vaspin (G , G') and GRP78 (H , H') . Representative microphotographs of control (A - H) and flutamide-treated testes ( A' - H' ). Additionally stained with Mayer's haematoxylin. Bars = 10 mum. Positive staining for chemerin, CCRL2, CMKLR1, apelin and APLNR are restricted to Leydig cells (arrows), whereas staining of GPR1, vaspin and GRP78 is visible in Leydig cells (arrows) and seminiferous tubules (open arrows). Note the decreased levels of chemerin ( A' ), GPR1 ( D' ), APLNR ( F' ) and vaspin ( G' ) and increased levels of CCRL2 ( B' ) and apelin ( E' ) intensities after flutamide administration. The intensity of CMKLR1 and GRP78 staining in flutamide ( C',H' ) and control ( C , H ) Leydig cells are similar. No immunopositive staining of chemerin, apelin and vaspin and their receptors was observed when the primary antibodies are omitted ( A - H , A' - H', insertion in upper left corner of microphotographs). Histograms ( I ) depicting staining intensities of chemerin, apelin, vaspin, and their receptors are expressed as relative optical density of the brown staining product in Leydig cells. The results are presented as means +- SD. Statistically significant differences are flagged with asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001). Control ( n = 6) and f

SERPINA12 Antibody in Western Blot (WB) — Figure 2 Representative Western blots assessing the relative expression of chemerin ( A ), CCRL2 ( B ), CMKLR1 ( C ), GPR1 ( D ) , apelin ( E ), APLNR (F), vaspin ( G ) and GRP78 ( H ) proteins in control and flutamide-treated testes. Normalisation was performed with beta-Actin as a loading control. Protein levels within control testes were given a value of 1. Data obtained from three separate analyses are presented as means +- SD. Statistically significant differences are flagged with asterisks (* p < 0.05, ** p < 0.01) between control ( n = 6) and flutamide-treated ( n = 6) animals; C: control, F: flutamide.

Product Details

PA5-30989

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 119 and 414 of Human SERPINA12 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.57 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2548463

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.

International journal of molecular sciences

Kurowska P,Mlyczyńska E,Estienne A,Barbe A,Rajska I,Soból K,Poniedziałek-Kempny K,Dupont J,Rak A

Published figure using SERPINA12 polyclonal antibody (Product # PA5-30989) in Immunocytochemistry

Tue Dec 08 00:00:00 EST 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Published figure using SERPINA12 polyclonal antibody (Product # PA5-30989) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Published figure using SERPINA12 polyclonal antibody (Product # PA5-30989) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Published figure using SERPINA12 polyclonal antibody (Product # PA5-30989) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

Flutamide Alters the Expression of Chemerin, Apelin, and Vaspin and Their Respective Receptors in the Testes of Adult Rats.

International journal of molecular sciences

Brzoskwinia M,Pardyak L,Rak A,Kaminska A,Hejmej A,Marek S,Kotula-Balak M,Bilinska B

Published figure using SERPINA12 polyclonal antibody (Product # PA5-30989) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

Vaspin in the pig ovarian follicles: expression and regulation by different hormones.

Reproduction (Cambridge, England)

Kurowska P,Mlyczyńska E,Barbe A,Staub C,Gregoraszczuk E,Dupont J,Rak A

PA5-30989 was used in Western Blot, Immunohistochemistry to show vaspin expression and regulation in the pig ovary, indicating vaspin as a new regulator in female reproduction.

Thu Aug 01 00:00:00 EDT 2019

Vaspin in the pig ovarian follicles: expression and regulation by different hormones.

Reproduction (Cambridge, England)

Kurowska P,Mlyczyńska E,Barbe A,Staub C,Gregoraszczuk E,Dupont J,Rak A

PA5-30989 was used in Western Blot, Immunohistochemistry to show vaspin expression and regulation in the pig ovary, indicating vaspin as a new regulator in female reproduction.

Thu Aug 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: a12; OL-64; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; Vaspin; Visceral adipose tissue-derived serine protease inhibitor; Visceral adipose-specific serpin

Gene Aliases: OL-64; SERPINA12

UniProt ID: (Human) Q8IW75

Entrez Gene ID: (Human) 145264

Molecular Function: protease inhibitor

glucose metabolic process negative regulation of endopeptidase activity positive regulation of phosphatidylinositol 3-kinase signaling negative regulation of gluconeogenesis positive regulation of insulin receptor signaling pathway negative regulation of lipid biosynthetic process regulation of cholesterol metabolic process regulation of triglyceride metabolic process

Process(es)

serine-type endopeptidase inhibitor activity