Proteintech

SESN3 Polyclonal Antibody

View all (10) SESN3 antibodies

Questions & Answers

Tech Support

Questions & Answers

Tech Support

Product Image Gallery

SESN3 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SESN3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

11431-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 5 publications 5 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SESN3 Fusion Protein Ag1961 (1-321 aa encoded by BC017296) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MNRGGGSPS AAANYLLCTN CRKVLRKDKR IRVSQPLTRG PSAFIPEKEV VQANTVDERT NFLVEEYSTS GRLDNITQVM SLHTQYLESF LRSQFYMLRM DGPLPLPYRH YIAIMAAARH QCSYLINMHV DEFLKTGGIA EWLNGLEYVP QRLKNLNEIN KLLAHRPWLI TKEHIQKLVK TGENNWSLPE LVHAVVLLAH YHALASFVFG SGINPERDPE ISNGFRLISV NNFCVCDLAN DNNIENASLS GSNFGIVDSL SELEALMERM KRLQEEREDE EASQEEMSTR FEKEKKESLF VVSGDTFHSF PHSGAFLHFF AF (1-321 aa encoded by BC017296)

Target Information

May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25263562). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exercise improves glucose uptake in murine myotubes through the AMPKα2-mediated induction of Sestrins.

Biochimica et biophysica acta. Molecular basis of disease

Wang T,Niu Y,Liu S,Yuan H,Liu X,Fu L

Mon Oct 01 00:00:00 EDT 2018

Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3.

Brain research

Huang LG,Zou J,Lu QC

Fri Jun 15 00:00:00 EDT 2018

Silencing rno-miR-155-5p in rat temporal lobe epilepsy model reduces pathophysiological features and cell apoptosis by activating Sestrin-3.

Brain research

Huang LG,Zou J,Lu QC

Fri Jun 15 00:00:00 EDT 2018

Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.

eLife

Nogueira V,Patra KC,Hay N

Tue Apr 24 00:00:00 EDT 2018

Regulatory effects of sestrin 3 (SESN3) in BCR-ABL expressing cells.

PloS one

Vakana E,Arslan AD,Szilard A,Altman JK,Platanias LC

Mon Jul 07 00:00:00 EDT 2014

FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.

Developmental cell

Chen CC,Jeon SM,Bhaskar PT,Nogueira V,Sundararajan D,Tonic I,Park Y,Hay N

Tue Apr 20 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Sestrin-3

Gene Aliases: 5630400E15Rik; BC003348; SESN3; SEST3

UniProt ID: (Human) P58005, (Mouse) Q9CYP7

Entrez Gene ID: (Human) 143686, (Rat) 315427, (Mouse) 75747

Molecular Function: peroxidase

oxidation-reduction process cellular response to amino acid stimulus regulation of response to reactive oxygen species negative regulation of TORC1 signaling response to insulin glucose homeostasis regulation of protein kinase B signaling

Process(es)

oxidoreductase activity molecular_function