Invitrogen

SET Polyclonal Antibody

View all (21) SET antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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SET Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SET in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115888) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

SET Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SET in human colorectal cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115888) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

SET Antibody in Western Blot (WB) — Western blot analysis of SET in rat muscle (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115888).

Product Details

PA5-115888

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human SET, corresponding to a region within N-terminal amino acids. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2900522

Target Information

SET is a multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HLA-DR-associated protein II; I IGAAD; I-2PP2A; IGAAD; Inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; Liver regeneration-related protein LRRGR00002; OTTHUMP00000022286; PHAPII; Phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; Protein SET; RP11-216B9.3; SET nuclear oncogene; SET translocation; SET translocation (myeloid leukemia-associated); TAF-1a; TAF-1b; TAF-I; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor

Gene Aliases: 2PP2A; Ab1-115; I2PP2A; IGAAD; IPP2A2; PHAPII; SET; TAF-I; TAF-IBETA

UniProt ID: (Human) Q01105, (Rat) Q63945

Entrez Gene ID: (Human) 6418, (Rat) 307947

Molecular Function: chromatin/chromatin-binding, or -regulatory protein