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SETD2 Antibody (PA5-34935) in WB
Western Blot using SETD2 Polyclonal Antibody (Product # PA5-34935). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD2 Polyclonal Antibody (Product # PA5-34935) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-34935
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contains a sequence corresponding to a region within amino acids 511 and 2564 of HYPB/SETD2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552284
Product Specific Information
Recommended positive controls: 293T, Jurkat.^M
^M
Predicted reactivity: Rat (84%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16420; FLJ22472; FLJ23184; FLJ45883; HIF-1; HIP-1; Histone-lysine N-methyltransferase SETD2; hSET2; huntingtin interacting protein 1; Huntingtin yeast partner B; Huntingtin-interacting protein 1; Huntingtin-interacting protein B; Lysine N-methyltransferase 3A; p231HBP; Protein-lysine N-methyltransferase SETD2; SET domain-containing protein 2
Gene Aliases: HBP231; HIF-1; HIF1; HIP-1; HSPC069; HYPB; KIAA1732; KMT3A; LLS; p231HBP; SET2; SETD2
UniProt ID: (Human) Q9BYW2
Entrez Gene ID: (Human) 29072
angiogenesis
morphogenesis of a branching structure
neural tube closure
mismatch repair
regulation of transcription, DNA-templated
transcription elongation from RNA polymerase II promoter
regulation of mRNA export from nucleus
forebrain development
nucleosome organization
cell migration involved in vasculogenesis
mesoderm morphogenesis
embryonic cranial skeleton morphogenesis
stem cell development
pericardium development
embryonic placenta morphogenesis
coronary vasculature morphogenesis
histone H3-K36 trimethylation
histone H3-K36 dimethylation
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