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Invitrogen
SETD7 Polyclonal Antibody
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FIGURE: 1 / 2
SETD7 Antibody (PA5-97320) in WB
Western blot analysis of SETD7 in extracts from normal (control) and SETD7 knockout (KO) 293T cells. Samples were incubated with SETD7 Polyclonal antibody (Product # PA5-97320) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-97320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_0851511)
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Amount
48 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK; Positive Samples: HeLa, HepG2, Mouse pancreas, Mouse brain, Rat brain; Cellular Location: Chromosome, Nucleus
Target Information
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ21193; H3-K4 methyltransferase; H3-K4-HMTase; H3-K4-HMTase SETD7; Histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; Histone-lysine N-methyltransferase SETD7; Lysine N-methyltransferase 7; OTTHUMP00000220049; SET domain containing 7, lysine methyltransferase; SET domain containing lysine methyltransferase 7; SET domain-containing protein 7; SET7; SET7/9; SET9
Gene Aliases: 1600028F23Rik; H3K4MT; KIAA1717; KMT7; mKIAA1717; SET7; SET7/9; SET9; SETD7
UniProt ID: (Human) Q8WTS6, (Mouse) Q8VHL1
Entrez Gene ID: (Human) 80854, (Rat) 689954, (Mouse) 73251
p53 binding
chromatin binding
protein binding
methyltransferase activity
protein-lysine N-methyltransferase activity
transferase activity
histone methyltransferase activity
histone H3 methyltransferase activity
histone H3K4 monomethyltransferase activity
molecular_function
histone-lysine N-methyltransferase activity
chromatin/chromatin-binding, or -regulatory protein
histone modifying enzyme
regulation of DNA repair
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
peptidyl-lysine monomethylation
peptidyl-lysine dimethylation
methylation
response to ethanol
positive regulation of transcription, DNA-templated
heterochromatin organization
response to alcohol
transcription, DNA-templated
biological_process
peptidyl-lysine methylation
histone lysine methylation
negative regulation of transferase activity
regulation of histone H3-K9 methylation
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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