Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
SETD8 Antibody (PA5-112135) in WB
Western Blot analysis of SETD8 using SETD8 Polyclonal Antibody (Product # PA5-112135) at a dilution of 1:500. Lane A: 293T whole cell lysate, Lane B: HCT-116 whole cell lysate, Lane C: U87-MG whole cell lysate, Lane D: K562 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 50 kDa.
Product Details
PA5-112135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human PR-Set7/KMT5A Protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866871
Product Specific Information
This product can be used in direct ELISA.
Target Information
SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: H4-K20-HMTase; H4-K20-HMTase KMT5A; H4-K20-HMTase SETD8; H4-K20-specific histone methyltransferase; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase KMT5A; histone-lysine N-methyltransferase SETD8; lysine (K)-specific methyltransferase 5A; Lysine N-methyltransferase 5A; Lysine-specific methylase 5A; N-lysine methyltransferase KMT5A; N-lysine methyltransferase SETD8; PR-Set7; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; SET domain containing (lysine methyltransferase) 8; SET domain-containing protein 8
Gene Aliases: KMT5A; PR-Set7; PRSET7; SET07; SET8; SETD8
UniProt ID: (Human) Q9NQR1
Entrez Gene ID: (Human) 387893
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
mitotic nuclear division
peptidyl-lysine monomethylation
histone H4-K20 methylation
regulation of DNA damage response, signal transduction by p53 class mediator
negative regulation of transcription, DNA-templated
cell division
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support