Proteintech

SETDB1 Polyclonal Antibody

Product Image Gallery

SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 11231-1-AP (SETDB1 antibody) at dilution of 1:50 (under 40x lens).

SETDB1 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SETDB1 Antibody in Immunoprecipitation (IP) — IP result of anti-SETDB1 (IP:11231-1-AP, 3ug; Detection:11231-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

Product Details

11231-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 12 publications 12 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
ChIP assay (ChIP)	Assay-dependent	View 12 publications 12 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SETDB1 Fusion Protein Ag1725 (1-350 aa encoded by BC009362) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa.

Immunogen sequence: MSSLPGCIG LDAATATVES EEIAELQQAV VEELGISMEE LRHFIDEELE KMDCVQQRKK QLAELETWVI QKESEVAHVD QLFDDASRAV TNCESLVKDF YSKLGLQYRD SSSEDESSRP TEIIEIPDED DDVLSIDSGD AGSRTPKDQK LREAMAALRK SAQDVQKFMD AVNKKSSSQD LHKGTLSQMS GELSKDGDLI VSMRILGKKR TKTWHKGTLI AIQTVGPGKK YKVKFDNKGK SLLSGNHIAY DYHPPADKLY VGSRVVAKYK DGNQVWLYAG IVAETPNVKN KLRFLIFFDD GYASYVTQSE LYPICRPLKK TWEDIEDISC RDFIEEYVTA YPNRPMVLLK S (1-350 aa encoded by BC009362)

Target Information

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.

Journal of Cancer

Chen K,Zhang F,Ding J,Liang Y,Zhan Z,Zhan Y,Chen LH,Ding Y

Thu Mar 17 00:00:00 EDT 2022

AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells.

Journal of molecular cell biology

Zhang Y,Wang C,Liu X,Yang Q,Ji H,Yang M,Xu M,Zhou Y,Xie W,Luo Z,Lin C

Thu Sep 19 00:00:00 EDT 2019

SETDB1 induces epithelial‑mesenchymal transition in breast carcinoma by directly binding with Snail promoter.

Oncology reports

Yang W,Su Y,Hou C,Chen L,Zhou D,Ren K,Zhou Z,Zhang R,Liu X

Fri Feb 01 00:00:00 EST 2019

SETDB1 is essential for mouse primordial germ cell fate determination by ensuring BMP signaling.

Development (Cambridge, England)

Mochizuki K,Tando Y,Sekinaka T,Otsuka K,Hayashi Y,Kobayashi H,Kamio A,Ito-Matsuoka Y,Takehara A,Kono T,Osumi N,Matsui Y

Mon Dec 03 00:00:00 EST 2018

SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.

Developmental cell

Hirota T,Blakeley P,Sangrithi MN,Mahadevaiah SK,Encheva V,Snijders AP,ElInati E,Ojarikre OA,de Rooij DG,Niakan KK,Turner JMA

Mon Dec 03 00:00:00 EST 2018

SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.

Developmental cell

Hirota T,Blakeley P,Sangrithi MN,Mahadevaiah SK,Encheva V,Snijders AP,ElInati E,Ojarikre OA,de Rooij DG,Niakan KK,Turner JMA

Mon Dec 03 00:00:00 EST 2018

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.

Nature microbiology

Yurkovetskiy L,Guney MH,Kim K,Goh SL,McCauley S,Dauphin A,Diehl WE,Luban J

Sat Dec 01 00:00:00 EST 2018

SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States.

Cell stem cell

Cossec JC,Theurillat I,Chica C,Búa Aguín S,Gaume X,Andrieux A,Iturbide A,Jouvion G,Li H,Bossis G,Seeler JS,Torres-Padilla ME,Dejean A

Thu Nov 01 00:00:00 EDT 2018

Melatonin protects mouse spermatogonial stem cells against hexavalent chromium-induced apoptosis and epigenetic histone modification.

Toxicology and applied pharmacology

Lv Y,Zhang P,Guo J,Zhu Z,Li X,Xu D,Zeng W

Thu Feb 01 00:00:00 EST 2018

Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway.

Biochimica et biophysica acta. Gene regulatory mechanisms

Liu T,Chen X,Li T,Li X,Lyu Y,Fan X,Zhang P,Zeng W

Sun Oct 01 00:00:00 EDT 2017

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.

Nature genetics

Tchasovnikarova IA,Timms RT,Douse CH,Roberts RC,Dougan G,Kingston RE,Modis Y,Lehner PJ

Sat Jul 01 00:00:00 EDT 2017

ATF7IP-Mediated Stabilization of the Histone Methyltransferase SETDB1 Is Essential for Heterochromatin Formation by the HUSH Complex.

Cell reports

Timms RT,Tchasovnikarova IA,Antrobus R,Dougan G,Lehner PJ

Tue Oct 11 00:00:00 EDT 2016

A Histone Methyltransferase ESET Is Critical for T Cell Development.

Journal of immunology (Baltimore, Md. : 1950)

Takikita S,Muro R,Takai T,Otsubo T,Kawamura YI,Dohi T,Oda H,Kitajima M,Oshima K,Hattori M,Endo TA,Toyoda T,Weis J,Shinkai Y,Suzuki H

Thu Sep 15 00:00:00 EDT 2016

Activation of Endogenous Retroviruses in Dnmt1(-/-) ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA.

Cell stem cell

Sharif J,Endo TA,Nakayama M,Karimi MM,Shimada M,Katsuyama K,Goyal P,Brind'Amour J,Sun MA,Sun Z,Ishikura T,Mizutani-Koseki Y,Ohara O,Shinkai Y,Nakanishi M,Xie H,Lorincz MC,Koseki H

Thu Jul 07 00:00:00 EDT 2016

Activation of Endogenous Retroviruses in Dnmt1(-/-) ESCs Involves Disruption of SETDB1-Mediated Repression by NP95 Binding to Hemimethylated DNA.

Cell stem cell

Sharif J,Endo TA,Nakayama M,Karimi MM,Shimada M,Katsuyama K,Goyal P,Brind'Amour J,Sun MA,Sun Z,Ishikura T,Mizutani-Koseki Y,Ohara O,Shinkai Y,Nakanishi M,Xie H,Lorincz MC,Koseki H

Thu Jul 07 00:00:00 EDT 2016

Hypomethylation of ERVs in the sperm of mice haploinsufficient for the histone methyltransferase Setdb1 correlates with a paternal effect on phenotype.

Scientific reports

Daxinger L,Oey H,Isbel L,Whitelaw NC,Youngson NA,Spurling A,Vonk KK,Whitelaw E

Tue Apr 26 00:00:00 EDT 2016

The histone methyltransferase SETDB1 represses endogenous and exogenous retroviruses in B lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America

Collins PL,Kyle KE,Egawa T,Shinkai Y,Oltz EM

Tue Jul 07 00:00:00 EDT 2015

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Science (New York, N.Y.)

Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,Göttgens B,Dougan G,Dawson MA,Lehner PJ

Fri Jun 26 00:00:00 EDT 2015

SETDB1 accelerates tumourigenesis by regulating the WNT signalling pathway.

The Journal of pathology

Sun QY,Ding LW,Xiao JF,Chien W,Lim SL,Hattori N,Goodglick L,Chia D,Mah V,Alavi M,Kim SR,Doan NB,Said JW,Loh XY,Xu L,Liu LZ,Yang H,Hayano T,Shi S,Xie D,Lin DC,Koeffler HP

Sun Mar 01 00:00:00 EST 2015

SETDB1 accelerates tumourigenesis by regulating the WNT signalling pathway.

The Journal of pathology

Sun QY,Ding LW,Xiao JF,Chien W,Lim SL,Hattori N,Goodglick L,Chia D,Mah V,Alavi M,Kim SR,Doan NB,Said JW,Loh XY,Xu L,Liu LZ,Yang H,Hayano T,Shi S,Xie D,Lin DC,Koeffler HP

Sun Mar 01 00:00:00 EST 2015

KMT1E-mediated chromatin modifications at the FcγRIIb promoter regulate thymocyte development.

Genes and immunity

Martin FJ,Xu Y,Lohmann F,Ciccone DN,Nicholson TB,Loureiro JJ,Chen T,Huang Q

Sun Mar 01 00:00:00 EST 2015

Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs.

Human molecular genetics

Cruvinel E,Budinetz T,Germain N,Chamberlain S,Lalande M,Martins-Taylor K

Mon Sep 01 00:00:00 EDT 2014

The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.

Cell death & disease

An J,Zhang X,Qin J,Wan Y,Hu Y,Liu T,Li J,Dong W,Du E,Pan C,Zeng W

Thu Apr 24 00:00:00 EDT 2014

The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.

Cell death & disease

An J,Zhang X,Qin J,Wan Y,Hu Y,Liu T,Li J,Dong W,Du E,Pan C,Zeng W

Thu Apr 24 00:00:00 EDT 2014

The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice.

Cell death & disease

An J,Zhang X,Qin J,Wan Y,Hu Y,Liu T,Li J,Dong W,Du E,Pan C,Zeng W

Thu Apr 24 00:00:00 EDT 2014

Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus.

The Journal of biological chemistry

Kera Y,Katoh Y,Ohta M,Matsumoto M,Takano-Yamamoto T,Igarashi K

Fri May 10 00:00:00 EDT 2013

Functional analysis of KAP1 genomic recruitment.

Molecular and cellular biology

Iyengar S,Ivanov AV,Jin VX,Rauscher FJ,Farnham PJ

Sun May 01 00:00:00 EDT 2011

ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes.

PloS one

Frietze S,O'Geen H,Blahnik KR,Jin VX,Farnham PJ

Wed Dec 08 00:00:00 EST 2010

SetDB1 contributes to repression of genes encoding developmental regulators and maintenance of ES cell state.

Genes & development

Bilodeau S,Kagey MH,Frampton GM,Rahl PB,Young RA

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: ERG-associated protein with a SET domain, ESET; ERG-associated protein with SET domain; ESET; H3-K9-HMTase 4; Histone H3-K9 methyltransferase 4; Histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SET domain ERG-associated histone methyltransferase; SET domain, bifurcated 1; tudor domain containing 21

Gene Aliases: AU022152; ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E; mKIAA0067; SETDB1; TDRD21

UniProt ID: (Human) Q15047

Entrez Gene ID: (Human) 9869, (Mouse) 84505, (Rat) 689883

Molecular Function: histone modifying enzyme