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SF1 Polyclonal Antibody

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SF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-SF1 polyclonal antibody (Product # PA5-41967).

SF1 Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-SF1 polyclonal antibody (Product # PA5-41967).

Product Details

PA5-41967

Applications	Tested Dilution	Publications
Western Blot (WB)	1.25 µg/mL	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	4-8 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the N-terminal of human SF1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607741

Product Specific Information

Peptide sequence: NATPLDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Target Information

Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of Intracerebroventricular Glycogen Phosphorylase Inhibitor CP-316,819 Infusion on Hypothalamic Glycogen Content and Metabolic Neuron AMPK Activity and Neurotransmitter Expression in Male Rat.

Journal of molecular neuroscience : MN

Ibrahim MMH,Bheemanapally K,Alhamami HN,Briski KP

PA5-41967 was used in Western Blotting to investigate whether glycogen-derived fuel controls basal and/or hypoglycemic patterns of VMN gluco-regulatory neuron energy stability and transmitter signaling.

Fri May 01 00:00:00 EDT 2020

Hindbrain Estrogen Receptor Regulation of Ventromedial Hypothalamic Glycogen Metabolism and Glucoregulatory Transmitter Expression in the Hypoglycemic Female Rat.

Neuroscience

Napit PR,Ali MH,Shakya M,Mandal SK,Bheemanapally K,Mahmood ASMH,Ibrahim MMH,Briski KP

PA5-41967 was used in Western Blotting to investigate the neural substrates for oestrogen regulation of glucose homeostasis using female rats.

Mon Jul 15 00:00:00 EDT 2019

Hindbrain estrogen receptor regulation of ventromedial hypothalamic glycogen metabolism and glucoregulatory transmitter expression in the hypoglycemic male rat.

Neuroscience

Ali MH,Napit PR,Mahmood ASMH,Bheemanapally K,Alhamami HN,Uddin MM,Mandal SK,Ibrahim MMH,Briski KP

PA5-41967 was used in Western Blotting to show that ERβ inhibits VMN glycogen synthase and stimulates phosphorylase protein expression.

Sat Jun 15 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: BBP; M ZFM1; Mammalian branch point-binding protein; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162

Gene Aliases: BBP; D11S636; MBBP; SF1; ZCCHC25; ZFM1; ZNF162

UniProt ID: (Human) Q15637

Entrez Gene ID: (Human) 7536

Molecular Function: RNA splicing factor

Function(s)

spliceosomal complex assembly mRNA 3'-splice site recognition mRNA splicing, via spliceosome transcription, DNA-templated regulation of transcription, DNA-templated male sex determination Leydig cell differentiation negative regulation of smooth muscle cell proliferation regulation of steroid biosynthetic process negative regulation of nucleic acid-templated transcription

Process(es)

transcription corepressor activity RNA binding protein binding zinc ion binding identical protein binding poly(A) RNA binding

SF1 Polyclonal Antibody

Product Details

PA5-41967

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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