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Invitrogen
SF1 Polyclonal Antibody
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FIGURE: 1 / 9
SF1 Antibody (PA5-79983) in ICC/IF
Immunocytochemistry analysis of splicing factor 1 using anti-splicing factor 1 antibody (Product # PA5-79983). splicing factor 1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-splicing factor 1 antibody (Product # PA5-79983) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The ... View More
Product Details
PA5-79983
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SK-OV-3 whole cell, human HepG2 whole cell, mouse NIH/3T3 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell. IHC: mouse lung tissue, rat lung tissue, human placenta tissue, human rectal cancer tissue, mouse small intestine tissue, rat small intestine tissue. ICC/IF: A431 cell. Flow: A431 cell.
Target Information
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BBP; M ZFM1; Mammalian branch point-binding protein; SF; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
Gene Aliases: BBP; D11S636; MBBP; MZFM; SF1; WBP4; ZCCHC25; ZFM1; Zfp162; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536, (Mouse) 22668, (Rat) 117855
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
mRNA processing
RNA splicing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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