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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200-1:2,000 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
3.19 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2808093 |
Immunogen sequence: SLESLDTSLF AKNPKSKGTK RDTERNKDIA FLEAQIYEYV EILGEQRHLT HENVQRKQAR TGEEREEEEE EQISESESED EENEIIYNPK NLPLGWDGKP IPYWLYKLHG LNINYNCEIC GNYTYRGPKA FQRHFAEWRH AHGMRCLGIP NTAHFANVTQ IEDAVSLWAK LKLQKASERW QPDTEEEYED SSGNVVNKKT YEDLKRQGLL; Positive Samples: B cells, A-549, HepG2, HeLa, HL-60, Mouse lung, Mouse thymus, Rat liver; Cellular Location: Nucleus speckle
This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: pre-mRNA splicing factor SF3a (60kD); SAP 61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; Splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa
Gene Aliases: 4930512K19Rik; 60kDa; LRRGT00093; PRP9; PRPF9; SAP61; SF3A3; SF3a60
UniProt ID: (Human) Q12874, (Mouse) Q9D554
Entrez Gene ID: (Human) 10946, (Mouse) 75062, (Rat) 313583
Molecular Function:
RNA splicing factor
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