Invitrogen

SFTPA1 Polyclonal Antibody

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SFTPA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SFTPA1 on paraffin-embedded mouse lung tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with SFTPA1 polyclonal antibody (Product# PA5-79986) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

SFTPA1 Antibody in Western Blot (WB) — Western blot analysis of SFTPA1 in Lane 1: mouse lung tissue lysate, Lane 2: mouse lung tissue lysate, Lane 3: rat lung tissue lysate, Lane 4: rat lung tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with SFTPA1 polyclonal antibody (Product # PA5-79986) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Product Details

PA5-79986

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-

Product Specifications
Species Reactivity	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of mouse SFTPA1. View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5mg BSA
Contains	0.05mg sodium azide, 0.05mg thimerosal
Storage conditions	-20°C
RRID	AB_2747101

Product Specific Information

The synthetic peptide sequence is 233-248aa, WNDKGCLQYRLAICEF

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Collectin 4; FLJ50593; FLJ51913; FLJ61144; FLJ77898; FLJ79095; FLJ99559; MGC133365; MGC198590; OTTHUMP00000019928; OTTHUMP00000019929; OTTHUMP00000019930; OTTHUMP00000019931; PSAP; PSP A; PSP-A; Pulmonary surfactant-associated protein A; SP A; SP A1; Surfactant; surfactant associated protein A; surfactant pulmonary associated protein A1; surfactant, pulmonary-associated protein A1; Surfactant-associated protein 1 (pulmonary surfactant protein SP-A); Surfactant-associated protein 1 (pulmonary surfactant protein, SP-A)

Gene Aliases: Sftp-1; Sftp1; Sftpa; Sftpa1; Sftpl; SP-A

UniProt ID: (Rat) P08427

Entrez Gene ID: (Rat) 24773, (Mouse) 20387

Molecular Function: surfactant