Invitrogen

SGCG Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (9) SGCG antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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SGCG Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of SGCG in human cell line U-251 MG using a SGCG Polyclonal Antibody (Product # PA5-52439) shows localization to nucleoplasm.

SGCG Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of SGCG in human heart muscle and prostate tissues using SGCG Polyclonal Antibody (Product # PA5-52439). Corresponding SGCG RNA-seq data are presented for the same tissues.

SGCG Antibody in Western Blot (WB) — Western blot analysis of SGCG in control (vector only transfected HEK293T lysate) and SGCG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SGCG Polyclonal Antibody (Product # PA5-52439).

SGCG Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of SGCG demonstrates antibody specificity. Immunohistochemical analysis of SGCG using anti-SGCG Polyclonal Antibody (Product # PA5-52439), shows significant staining of SGCG in human heart muscle and shows minimal or weak staining in human prostate tissues. The relative expression levels of SGCG within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-52439

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:20-1:50	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human SGCG View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2647209

Product Specific Information

Immunogen sequence: YAKEIHSRVD SSLLLQSTQN VTVNARNSEG EVTGRLKVGP KMVEVQNQQF QINSNDGKPL FTVDEKEVVV GTDKLRVTGP EGALFEHSVE TPLVRADPFQ D

Highest antigen sequence identity to the following orthologs: Mouse - 85%, Rat - 83%.

Target Information

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 35 kDa dystrophin-associated glycoprotein; 35DAG; 35kD dystrophin-associated glycoprotein; Gamma-sarcoglycan; Gamma-SG; sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein); TYPE

Gene Aliases: 35DAG; A4; DAGA4; DMDA; DMDA1; gamma-SG; LGMD2C; MAM; SCARMD2; SCG3; SGCG

UniProt ID: (Human) Q13326

Entrez Gene ID: (Human) 6445

Molecular Function: cytoskeletal protein