Invitrogen

SGPL1 Polyclonal Antibody

Product Image Gallery

SGPL1 Antibody — Fig 3 Rescue of HeLa Delta SGPL1 cells by exogenous Flag-SGPL1. HeLa wildtype, HeLa Delta SGPL1 and HeLa Delta SGPL1 transfected with Flag-tagged SGPL1 were labeled with 6 muM pacSph for 4 h. Upper Panel: Proteins precipitated during the lipid extraction were solubilized, analyzed by SDS-PAGE and immunoblotting, using anti-FLAG antibodies (red). Detection of endogenous calnexin with anti-calnexin antibody (CNX, green) was used as a loading control. Lower Panel: Extracted lipids were subjected to click reaction with fluorogenic coumarin azide, separated by TLC and exited with UV light. Lipids were identified via with alkyne lipid standards ( S3 Fig ).

SGPL1 Antibody in Western Blot (WB) — Western blot detection of the ~ 64 kDa S1P lyase 1 protein from MDBK cell lysate using Product # PA1-12722.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:400

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to sphingosine 1-phosphate lyase 1.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2285994

Product Specific Information

By Western blot, PA1-12722 detects a ~64 kDa protein representing S1P lyase 1 from MDBK cell lysate.

Target Information

Sphingosine-1-phosphate lyase cleaves phosphorylated sphingoid bases, such as sphingosine-1 phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. The catalytic activity of sphingosine 1- phosphate lyase converts sphingosine 1-phosphate to phosphoethanolamine and palmitaldehyde using Pyridoxal phosphate as a co-factor. Sphingosine 1-phosphate lyase is a type III membrane protein found in the endoplasmic reticulum. Sphingosine 1-phosphate lyase is found primarily in the liver and kidney. Sphingosine 1-phosphate lyase belongs to the group II decarboxylase family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AI428538; D10Xrf456; hSPL; mSPL; S1PL; SP-lyase 1; sphingosine phosphate lyase 1; Sphingosine-1-phosphate aldolase; Sphingosine-1-phosphate lyase 1; Spl; SPL 1

Gene Aliases: AI428538; BOS_24203; D10Xrf456; KIAA1252; S1PL; SGPL1; Spgl1; SPL

UniProt ID: (Human) O95470, (Mouse) Q8R0X7, (Rat) Q8CHN6

Entrez Gene ID: (Human) 8879, (Bovine) 522515, (Mouse) 20397, (Rat) 286896

Molecular Function: decarboxylase lyase