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Invitrogen
SHANK2 Polyclonal Antibody
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FIGURE: 1 / 4
SHANK2 Antibody (PA5-78652) in ICC/IF
SHANK2 Polyclonal Antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 Polyclonal Antibody (Product # PA5-78652) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-78652
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: mouse brain, rat brain
Predicted Reactivity: Human (86%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cortactin-binding protein 1; CortBP1; CORTBP1CTTNBP1; GKAP/SAPAP-interacting protein; proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains protein 2; SH3/ankyrin domain gene 2; Shank2; SPANK-3
Gene Aliases: CortBP1; Kiaa1022; mKIAA1022; ProSAP1; Shank2; Spank-3
UniProt ID: (Mouse) Q80Z38, (Rat) Q9QX74
Entrez Gene ID: (Mouse) 210274, (Rat) 171093
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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