Invitrogen

SHANK3 Polyclonal Antibody

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SHANK3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of human brain tissue using a SHANK3 polyclonal antibody (Product # PA5-34383) at a 25 µg/mL dilution.

SHANK3 Antibody in Western Blot (WB) — Western blot analysis of 3T3 cell lysate using a SHANK3 polyclonal antibody (Product # PA5-34383) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

20 µg/mL

Immunofluorescence (IF)

2.5 µg/mL

Immunohistochemistry (IHC)

2.5 µg/mL

Western Blot (WB)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 19 amino acid synthetic peptide near the center of human SHANK3

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_2551735

Product Specific Information

A suggested positive control is NIH-3T3 cell lysate.

PA5-34383 can be used with blocking peptide PEP-1426.

Target Information

SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; ProSAP2; PSAP2; SH3 and multiple ankyrin repeat domains 3; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; shank postsynaptic density protein; SHANK3; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; SPANK-2

Gene Aliases: AI841104; DEL22q13.3; KIAA1650; PROSAP2; PSAP2; SCZD15; SHANK3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-2

UniProt ID: (Human) Q9BYB0, (Mouse) Q4ACU6

Entrez Gene ID: (Human) 85358, (Mouse) 58234

Function(s)