Invitrogen

SHIP2 Polyclonal Antibody

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SHIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SHIP2 in paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with SHIP2 polyclonal antibody (Product # PA5-95562) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

SHIP2 Antibody in Western Blot (WB) — Western blot analysis of SHIP2 in Lane 1: human placenta tissue lysates, Lane 2: human HeLa whole cell lysate, Lane 3: human U20S whole cell lysate, Lane 4: human PC-3 whole cell lysate, Lane 5: human Caco-2 whole cell lysate, Lane 6: human A549 whole cell lysate, Lane 7: human K562 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with SHIP2 polyclonal antibody (Product # PA5-95562) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Product Details

PA5-95562

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
ELISA (ELISA)	0.1-0.5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human INPPL1 recombinant protein (Position: R1172-K1258). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807364

Product Specific Information

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

The protein encoded by this gene is an SH2-containing 5'-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodeling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 51C protein; AblSH3-binding protein; inositol polyphosphate phosphatase-like 1; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2; phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2; Protein 51C; SH2 domain-containing inositol 5'-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SH2 domain-containing inositol-5'-phosphatase 2; SH2-containing inositol phosphatase 2; SHIP-2

Gene Aliases: 51C; INPPL1; OPSMD; SHIP2

UniProt ID: (Human) Q13578, (Rat) Q9WVR3, (Mouse) Q6P549

Entrez Gene ID: (Human) 3636, (Rat) 65038, (Mouse) 16332

Function(s)