|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 483 of Human SHMT2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-32228 targets SHMT2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-32228 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 483 of Human SHMT2.
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mitochondrial dysfunction remodels one-carbon metabolism in human cells.
PA5-32228 was used in western blot to study remodeling of one-carbon metabolism in human cells via mitochondrial dysfunction
|Bao XR,Ong SE,Goldberger O,Peng J,Sharma R,Thompson DA,Vafai SB,Cox AG,Marutani E,Ichinose F,Goessling W,Regev A,Carr SA,Clish CB,Mootha VK||eLife (5:null)||2016|