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SHP1 Polyclonal Antibody

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SHP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 24546-1-AP (SHP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

SHP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24546-1-AP ( SHP1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SHP1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SHP1 Antibody in Immunoprecipitation (IP) — IP result of anti-SHP1 (IP:24546-1-AP, 4ug; Detection:24546-1-AP 1:600) with Jurkat cells lysate 3600ug.

Product Details

24546-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SHP1 Fusion Protein Ag21415 (506-598 aa encoded by BC002523)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: QYKFIYVAI AQFIETTKKK LEVLQSQKGQ ESEYGNITYP PAMKNAHAKA SRTSSKHKED VYENLHTKNK REEKVKKQRS ADKEKSKGSL KRK (506-598 aa encoded by BC002523)

Target Information

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 EDT 2019

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Oxidative medicine and cellular longevity

Maryam A,Mehmood T,Yan Q,Li Y,Khan M,Ma T

Tue Sep 18 00:00:00 EDT 2018

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 EST 2017

Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xiang M,Su H,Hong Z,Yang T,Shu G

Mon Jun 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6

Gene Aliases: HCP; HCPH; HPTP1C; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1

UniProt ID: (Human) P29350

Entrez Gene ID: (Human) 5777

Molecular Function: protein phosphatase

Function(s)

hematopoietic progenitor cell differentiation negative regulation of humoral immune response mediated by circulating immunoglobulin protein dephosphorylation apoptotic process G-protein coupled receptor signaling pathway cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cell differentiation platelet activation platelet formation T cell costimulation abortive mitotic cell cycle positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine dephosphorylation intracellular signal transduction megakaryocyte development negative regulation of T cell proliferation natural killer cell mediated cytotoxicity negative regulation of MAP kinase activity regulation of B cell differentiation negative regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway negative regulation of T cell receptor signaling pathway leukocyte migration regulation of release of sequestered calcium ion into cytosol regulation of type I interferon-mediated signaling pathway regulation of ERK1 and ERK2 cascade platelet aggregation regulation of G1/S transition of mitotic cell cycle

Process(es)

phosphotyrosine binding protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding SH3 domain binding protein kinase binding SH2 domain binding

SHP1 Polyclonal Antibody

Product Details

24546-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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