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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SI Antibodies

          Invitrogen

          SI Polyclonal Antibody

          View all (5) SI antibodies

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          Cite SI Polyclonal Antibody

          • Antibody Testing Data (4)
          SI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          SI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          SI Antibody (PA5-95609) in IHC (P)

          Immunohistochemistry (Paraffin) analysis of SI in paraffin-embedded section of mouse small intestine tissue using SI Polyclonal Antibody (Product # PA5-95609). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SI Antibody in Western Blot (WB)
          SI Antibody in Flow Cytometry (Flow)
          SI Polyclonal Antibody

          Product Details

          PA5-95609

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence of human SI (FQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDID).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807411

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: rat small intestine tissue. IHC: mouse small intestine tissue, rat small intestine tissue. Flow: CACO-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: alpha-glucosidase; oligosaccharide alpha-1,6-glucosidase; sucrase isomaltase, structural; sucrase-isomaltase; sucrase-isomaltase (alpha-glucosidase); Sucrase-isomaltase, intestinal

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          Gene Aliases: 2010204N08Rik; SI; Si-s; SUCIMAL

          View more View less

          UniProt ID: (Human) P14410

          View more View less

          Entrez Gene ID: (Human) 6476, (Rat) 497756, (Mouse) 69983

          View more View less

          Function(s)
          alpha-1,4-glucosidase activity oligo-1,6-glucosidase activity sucrose alpha-glucosidase activity carbohydrate binding hydrolase activity, hydrolyzing O-glycosyl compounds beta-fructofuranosidase activity protein binding glucosidase
          Process(es)
          carbohydrate metabolic process polysaccharide digestion aging response to nutrient response to sucrose response to fructose response to insulin response to vitamin A response to starvation response to glucocorticoid
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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