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Invitrogen
SIAH2 Polyclonal Antibody
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Product Details
PA5-36302
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 241-290 of human Siah-2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 38 kDa.
Purity is >95% by SDS-PAGE.
Target Information
SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 2; Seven in absentia homolog 2; Siah-2
Gene Aliases: AA415433; hSiah2; SIAH2; Sinh2
UniProt ID: (Human) O43255, (Mouse) Q06986, (Rat) Q8R4T2
Entrez Gene ID: (Human) 6478, (Mouse) 20439, (Rat) 140593
transcription corepressor activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
ubiquitin conjugating enzyme binding
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
ubiquitin-dependent protein catabolic process
apoptotic process
small GTPase mediated signal transduction
protein ubiquitination
regulation of protein ubiquitination
regulation of circadian rhythm
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
rhythmic process
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
amyloid fibril formation
regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway
cell cycle
multicellular organism development
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
cellular protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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