Invitrogen

SIGLEC15 Polyclonal Antibody

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Tech Support

Antibody Testing Data (2)
Published Figures (5)

SIGLEC15 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 7

SIGLEC15 Antibody (PA5-72765) in IHC

Immunohistochemistry of SIGLEC15 in human kidney tissue with SIGLEC15 Polyclonal Antibody (Product # PA5-72765) at 2.5 µg/mL.

Product Image Gallery

SIGLEC15 Antibody in Western Blot (WB) — Western Blot analysis of SIGLEC15 in human kidney tissue lysate with SIGLEC15 Polyclonal Antibody (Product # PA5-72765) at (A) 1 and (B) 2 µg/mL.

SIGLEC15 Antibody in Flow Cytometry (Flow) — Figure 4 TGF-beta1 upregulated Siglec-15 expression in HCC cells. ( A ) Western blot analysis of Siglec-15 protein levels in the cells treated with TGF-beta1 (50 ng/mL) for 24 h in the absence or presence of 8 mM SB431542 for 12 h. ( B ) Flow cytometric analysis of Siglec-15 expression in the HepG2 and Huh7 cells with the same treatment as specified above. * p < 0.05; ** p < 0.01. NC stands for untreated control. ( C ) Quantitative real-time PCR analysis of Siglec-15 mRNA expression in the same treated cells. ( D ) Dual-luciferase reporter assay for TGF-beta1 sensitivity in HCC cells. Cells were transfected with the luciferase reporter vector and then treated with or without 50 ng/mL TGF-beta1 for 24 h in the absence or presence of 8 mM SB431542 for 12 h.

SIGLEC15 Antibody in Immunocytochemistry (ICC/IF) — Figure 4 Double-immunofluorescent staining of CD68 and Siglec-15. CD68 staining ( A green). Siglec-15 staining ( B red). DAPI staining ( C blue). Merged images ( D ). Representative images of PCNSL peritumoral tissue with the co-expression of CD68 and Siglec-15. (magnification x 1000).

Product Details

PA5-72765

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0-2.0 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIGLEC15 antibody for a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is within amino acids 50 - 100 of SIGLEC15.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2718619

Target Information

Binds sialylated glycoproteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptional Regulation of Siglec-15 by ETS-1 and ETS-2 in Hepatocellular Carcinoma Cells.

International journal of molecular sciences

Sheng K,Wu Y,Lin H,Fang M,Xue C,Lin X,Lin X

PA5-72765 was used in Flow cytometry/Cell sorting to define a detailed molecular profile of how Siglec-15 is transcriptionally regulated which may offer significant opportunity for therapeutic intervention on HCC immunotherapy.

Mon Jan 02 00:00:00 EST 2023

Transcriptional Regulation of Siglec-15 by ETS-1 and ETS-2 in Hepatocellular Carcinoma Cells.

International journal of molecular sciences

Sheng K,Wu Y,Lin H,Fang M,Xue C,Lin X,Lin X

Mon Jan 02 00:00:00 EST 2023

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing, and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis.

Frontiers in immunology

Zhao Y,Lv J,Zhang H,Xie J,Dai H,Zhang X

PA5-72765 was used in Immunohistochemistry (Paraffin) to analyse gene expression profiles of six patients with PVNS, 11 patients with osteoarthritis (OA), nine patients with rheumatoid arthritis (RA) (E-MTAB-6141), and three healthy subjects (GSE143514).

Mon Oct 25 00:00:00 EDT 2021

Gene Expression Profiles Analyzed Using Integrating RNA Sequencing, and Microarray Reveals Increased Inflammatory Response, Proliferation, and Osteoclastogenesis in Pigmented Villonodular Synovitis.

Frontiers in immunology

Zhao Y,Lv J,Zhang H,Xie J,Dai H,Zhang X

Mon Oct 25 00:00:00 EDT 2021

Sialic acid-binding immunoglobulin-like lectin-15 expression on peritumoral macrophages is a favorable prognostic factor for primary central nervous system lymphoma patients.

Scientific reports

Fudaba H,Momii Y,Hirakawa T,Onishi K,Asou D,Matsushita W,Kawasaki Y,Sugita K,Fujiki M

PA5-72765 was used in Immunohistochemistry to indicate that the expression of Siglec-15 on peritumoral macrophages induces a favorable outcome in PCNSL patients.

Wed Jan 13 00:00:00 EST 2021

Sialic acid-binding immunoglobulin-like lectin-15 expression on peritumoral macrophages is a favorable prognostic factor for primary central nervous system lymphoma patients.

Scientific reports

Fudaba H,Momii Y,Hirakawa T,Onishi K,Asou D,Matsushita W,Kawasaki Y,Sugita K,Fujiki M

PA5-72765 was used in Immunohistochemistry to indicate that the expression of Siglec-15 on peritumoral macrophages induces a favorable outcome in PCNSL patients.

Wed Jan 13 00:00:00 EST 2021

Sialic acid-binding immunoglobulin-like lectin-15 expression on peritumoral macrophages is a favorable prognostic factor for primary central nervous system lymphoma patients.

Scientific reports

Fudaba H,Momii Y,Hirakawa T,Onishi K,Asou D,Matsushita W,Kawasaki Y,Sugita K,Fujiki M

PA5-72765 was used in Immunohistochemistry to indicate that the expression of Siglec-15 on peritumoral macrophages induces a favorable outcome in PCNSL patients.

Wed Jan 13 00:00:00 EST 2021

The significance of Siglec-15 expression in resectable non-small cell lung cancer.

Neoplasma

Hao JQ,Nong JY,Zhao D,Li HY,Su D,Zhou LJ,Dong YJ,Zhang C,Che NY,Zhang SC,Lin JZ,Yang JB,Zhang HT,Wang JH

PA5-72765 was used in Immunohistochemistry to investigate the significance of S15 expression with the clinicopathological and prognosis of patients with NSCLC and TILs.

Sun Nov 01 00:00:00 EDT 2020

Expression signature, prognosis value, and immune characteristics of Siglec-15 identified by pan-cancer analysis.

Oncoimmunology

Li B,Zhang B,Wang X,Zeng Z,Huang Z,Zhang L,Wei F,Ren X,Yang L

PA5-72765 was used in Immunohistochemistry to understand Siglec-15 expression signature, prognosis value, immune infiltration pattern, and biological function using multi-omic bioinformatics from public databases and verify them in lung cancer patients.

Fri Aug 28 00:00:00 EDT 2020

Expression signature, prognosis value, and immune characteristics of Siglec-15 identified by pan-cancer analysis.

Oncoimmunology

Li B,Zhang B,Wang X,Zeng Z,Huang Z,Zhang L,Wei F,Ren X,Yang L

Fri Aug 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CD33 antigen-like 3; CD33 molecule-like 3; sialic acid binding Ig-like lectin 15; Sialic acid-binding Ig-like lectin 15; Siglec; Siglec 15; Siglec-15

Gene Aliases: CD33L3; EG620235; HsT1361; RGD1565948; SIGLEC-15; SIGLEC-I; SIGLEC15

UniProt ID: (Human) Q6ZMC9

Entrez Gene ID: (Human) 284266, (Rat) 498888, (Mouse) 620235

Function(s)

regulation of actin cytoskeleton organization innate immune response regulation of bone resorption cellular response to lipoprotein particle stimulus regulation of osteoclast development

Process(es)

protein binding

SIGLEC15 Polyclonal Antibody

SIGLEC15 Antibody (PA5-72765) in IHC

Product Details

PA5-72765

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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