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Invitrogen
SIGLEC5 Polyclonal Antibody
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FIGURE: 1 / 4
SIGLEC5 Antibody (PA5-47058) in IHC (P)
Immunohistochemical analysis of SIGLEC5 in immersion fixed paraffin-embedded sections of human tonsil tissue. Samples were incubated with SIGLEC5 polyclonal antibody (Product # PA5-47058) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using... View More
Product Details
PA5-47058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-5 Glu17-Thr434
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 1% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD170; CD33 antigen-like 2; OB-binding protein 2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin 5; sialic acid-binding immunoglobulin-like lectin 5; Siglec; Siglec-5
Gene Aliases: CD170; CD33L2; OB-BP2; OBBP2; SIGLEC-5; SIGLEC5
UniProt ID: (Human) O15389
Entrez Gene ID: (Human) 8778
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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