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Proteintech
SIRP alpha Polyclonal Antibody
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Product Details
14482-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
SIRP alpha Fusion Protein Ag5865 (158-508 aa encoded by BC033092)
Conjugate
Form
Liquid
Concentration
0.13 mg/mL
Amount
19.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: ARATPQHTV SFTCESHGFS PRDITLKWFK NGNELSDFQT NVDPVGESVS YSIHSTAKVV LTREDVHSQV ICEVAHVTLQ GDPLRGTANL SETIRVPPTL EVTQQPVRAE NQVNVTCQVR KFYPQRLQLT WLENGNVSRT ETASTVTENK DGTYNWMSWL LVNVSAHRDD VKLTCQVEHD GQPAVSKSHD LKVSAHPKEQ GSNTAAENTG SNERNIYIVV GVVCTLLVAL LMAALYLVRI RQKKAQGSTS STRLHEPEKN AREITQVQSL DTNDITYADL NLPKGKKPAP QAAEPNNHTE YASIQTSPQP ASEDTLTYAD LDMVHLNRTP KQPAPKPEPS FSEYASVQVP RK (158-508 aa encoded by BC033092)
Target Information
SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bit; Brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like protein with tyrosine - based activation motifs; brain immunological-like with tyrosine-based motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172 alpha; CD172 antigen-like family member A; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; mSIRP-alpha1; MyD-1 antigen; p84; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); protein tyrosine phosphatase, non-receptor type substrate 1; Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); SHP substrate 1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRP-alpha1; SIRPalpha; SIRPalpha2; tyrosine phosphatase SHP substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1; unnamed protein product
Gene Aliases: AI835480; BIT; CD172A; MFR; MYD-1; MYD1; P84; PTPNS1; SHP-1; SHPS-1; SHPS1; SIRP; SIRPA
UniProt ID: (Human) P78324, (Rat) P97710, (Mouse) P97797
Entrez Gene ID: (Human) 140885, (Rat) 25528, (Mouse) 19261
protein phosphatase inhibitor activity
protein binding
SH3 domain binding
protein phosphatase binding
GTPase regulator activity
protein binding involved in heterotypic cell-cell adhesion
protein binding involved in cell-cell adhesion
protein antigen binding
protein tyrosine kinase binding
protein phosphorylated amino acid binding
transmembrane signal receptor
cell adhesion
regulation of gene expression
cell migration
negative regulation of lipopolysaccharide-mediated signaling pathway
regulation of interferon-gamma production
regulation of interleukin-1 beta production
regulation of interleukin-6 production
regulation of tumor necrosis factor production
negative regulation of interferon-beta production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
heterotypic cell-cell adhesion
monocyte extravasation
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of nitric oxide biosynthetic process
regulation of nitric oxide biosynthetic process
negative regulation of JNK cascade
negative regulation of inflammatory response
negative regulation of phagocytosis
positive regulation of phagocytosis
positive regulation of T cell activation
cellular response to hydrogen peroxide
negative regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-12
negative regulation of macrophage inflammatory protein 1 alpha production
negative regulation of chemokine (C-C motif) ligand 5 production
cell-cell adhesion
negative regulation of cytokine production involved in inflammatory response
positive regulation of reactive oxygen species metabolic process
hematopoietic progenitor cell differentiation
phagocytosis, recognition
phagocytosis, engulfment
cytoskeleton organization
actin filament organization
cell-matrix adhesion
movement of cell or subcellular component
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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