A suggested positive control is mouse liver tissue lysate.
PA5-20964 can be used with blocking peptide PEP-1078.
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
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Protein Aliases: 75SirT1; hSIR2; hSIRT1; mSIR2a; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; Regulatory protein SIR2 homolog 1; RP11-57G10.3, SIR2L1; Sir2; sir2-like 1; SIR2-like protein 1; SIR2alpha; SirtT1 75 kDa fragment; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; sirtuin type 1
Gene Aliases: AA673258; SIR2; Sir2a; SIR2alpha; SIR2L1; SIRT1