PA3-200 detects human and rat SIRT2 and shows no cross-reactivity to other SIRT isoforms.
PA3-200 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~48 kDa protein representing SIRT2 from SAOS2 cells. PA3-200 can be used for immunocytochemistry procedures.
PA3-200 immunizing peptide corresponds to amino acid residues 341-352 from the human SIRT2 protein. This sequence is completely conserved between human and rat.
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise.
SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
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Protein Aliases: 5E5 antigen; mSIR2L2; NAD-dependent deacetylase sirtuin-2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; silent information regulator 2; silent mating type information regulation 2, (S.cerevisiae, homolog)-like; sirtuin 2; SIR2-like protein 2; sir2-related protein type 2; Sirtuin for Silent Mating Type Information 2-Homolog; sirtuin type 2; sirtuin-2
Gene Aliases: 5730427M03Rik; SIR2; SIR2L; SIR2L2; SIRT2