|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: D(341) E A R T T E R E K P Q(352)|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-200 detects human and rat SIRT2 and shows no cross-reactivity to other SIRT isoforms.
PA3-200 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~48 kDa protein representing SIRT2 from SAOS2 cells. PA3-200 can be used for immunocytochemistry procedures.
PA3-200 immunizing peptide corresponds to amino acid residues 341-352 from the human SIRT2 protein. This sequence is completely conserved between human and rat.
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise.
SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A role for SIRT2-dependent histone H3K18 deacetylation in bacterial infection.
PA3-200 was used in ChIP assay and immunocytochemistry to study SIRT2-mediated epigenetic reprograming via histone H3K18 deacetylation during Listeria monocytogenes infection
|Eskandarian HA,Impens F,Nahori MA,Soubigou G,Coppée JY,Cossart P,Hamon MA||Science (New York, N.Y.) (341:null)||2013|
Adipose tissue-derived cells improve cardiac function following myocardial infarction.
PA3-200 was used in immunohistochemistry to study the therapeutic potential of adipose tissue-derived cells for the treatment of myocardial infarction
|Schenke-Layland K,Strem BM,Jordan MC,Deemedio MT,Hedrick MH,Roos KP,Fraser JK,Maclellan WR||The Journal of surgical research (153:217)||2009|
Alcohol-induced alterations in hepatic microtubule dynamics can be explained by impaired histone deacetylase 6 function.
PA3-200 was used in western blot to study the mechanism for the effect of alcohol on hepatic microtubule dynamics
|Shepard BD,Joseph RA,Kannarkat GT,Rutledge TM,Tuma DJ,Tuma PL||Hepatology (Baltimore, Md.) (48:1671)||2008|
Osthole regulates enzyme protein expression of CYP7A1 and DGAT2 via activation of PPARalpha/gamma in fat milk-induced fatty liver rats.
PA3-200 was used in western blot to study the effect of osthole on CYP7A1 and DGAT2 activity and its mechanism
|Zhang Y,Xie ML,Xue J,Gu ZL||Journal of Asian natural products research (10:807)||2008|