Proteintech

SIRT3 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SIRT3 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

10099-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 9 publications 9 publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SIRT3 Fusion Protein Ag0148 (202-399 aa encoded by BC001042) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: GNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK (202-399 aa encoded by BC001042)

Target Information

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sirtuin 3-dependent mitochondrial redox homeostasis protects against AGEs-induced intervertebral disc degeneration.

Redox biology

Song Y,Li S,Geng W,Luo R,Liu W,Tu J,Wang K,Kang L,Yin H,Wu X,Gao Y,Zhang Y,Yang C

Mon Oct 01 00:00:00 EDT 2018

Salidroside attenuates endothelial cellular senescence via decreasing the expression of inflammatory cytokines and increasing the expression of SIRT3.

Mechanisms of ageing and development

Xing SS,Li J,Chen L,Yang YF,He PL,Li J,Yang J

Mon Oct 01 00:00:00 EDT 2018

Modulation of mitochondrial phenotypes by endurance exercise contributes to neuroprotection against a MPTP-induced animal model of PD.

Life sciences

Jang Y,Kwon I,Song W,Cosio-Lima LM,Taylor S,Lee Y

Sat Sep 15 00:00:00 EDT 2018

Cryptotanshinone suppresses cell proliferation and glucose metabolism via STAT3/SIRT3 signaling pathway in ovarian cancer cells.

Cancer medicine

Yang Y,Cao Y,Chen L,Liu F,Qi Z,Cheng X,Wang Z

Sat Sep 01 00:00:00 EDT 2018

Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Journal of molecular medicine (Berlin, Germany)

Sun Y,Tian Z,Liu N,Zhang L,Gao Z,Sun X,Yu M,Wu J,Yang F,Zhao Y,Ren H,Chen H,Zhao D,Wang Y,Dong S,Xu C,Lu F,Zhang W

Sun Apr 01 00:00:00 EDT 2018

Protective effects of dioscin against fructose-induced renal damage via adjusting Sirt3-mediated oxidative stress, fibrosis, lipid metabolism and inflammation.

Toxicology letters

Qiao Y,Xu L,Tao X,Yin L,Qi Y,Xu Y,Han X,Tang Z,Ma X,Liu K,Peng J

Thu Mar 01 00:00:00 EST 2018

Protective effects of dioscin against fructose-induced renal damage via adjusting Sirt3-mediated oxidative stress, fibrosis, lipid metabolism and inflammation.

Toxicology letters

Qiao Y,Xu L,Tao X,Yin L,Qi Y,Xu Y,Han X,Tang Z,Ma X,Liu K,Peng J

Thu Mar 01 00:00:00 EST 2018

Impaired mitochondrial fusion, autophagy, biogenesis and dysregulated lipid metabolism is associated with preeclampsia.

Experimental cell research

Zhou X,Han TL,Chen H,Baker PN,Qi H,Zhang H

Sun Oct 01 00:00:00 EDT 2017

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Nature communications

Liu M,Liang K,Zhen J,Zhou M,Wang X,Wang Z,Wei X,Zhang Y,Sun Y,Zhou Z,Su H,Zhang C,Li N,Gao C,Peng J,Yi F

Mon Sep 04 00:00:00 EDT 2017

Role of Sirtuin3 in high glucose-induced apoptosis in renal tubular epithelial cells.

Biochemical and biophysical research communications

Jiao X,Li Y,Zhang T,Liu M,Chi Y

Fri Nov 18 00:00:00 EST 2016

Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity.

Anatomy & cell biology

Yoon SP,Kim J

Thu Sep 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3

Gene Aliases: 2310003L23Rik; AI848213; SIR2L3; SIRT3

UniProt ID: (Human) Q9NTG7, (Mouse) Q8R104

Entrez Gene ID: (Human) 23410, (Mouse) 64384, (Rat) 293615

Function(s)

protein ADP-ribosylation protein deacetylation mitochondrion organization aerobic respiration peptidyl-lysine deacetylation histone H3 deacetylation negative regulation of peptidyl-lysine acetylation positive regulation of insulin secretion negative regulation of ERK1 and ERK2 cascade negative regulation of reactive oxygen species metabolic process aging

Process(es)

SIRT3 Polyclonal Antibody

Product Details

10099-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!