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Invitrogen
SIRT3 Polyclonal Antibody
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FIGURE: 1 / 2
SIRT3 Antibody (PA5-95202) in WB
Western blot analysis of SIRT3 in Lane 1: rat spleen tissue lysate (50 µg), Lane 2: human placenta tissue lysate (50 µg), Lane 3: HeLa whole cell lysate (40 µg), Lane 4: COLO320 whole cell lysate (40 µg). Samples were incubated with SIRT3 polyclonal antibody (Product # PA5-95202).
Product Details
PA5-95202
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: P66-K399).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat spleen tissue, human placenta tissue, human Hela whole cell, human COLO320 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3; unnamed protein product; yeast silent information regulator 2 (Sir2p) homolog
Gene Aliases: SIR2L3; SIRT3
UniProt ID: (Human) Q9NTG7
Entrez Gene ID: (Human) 23410, (Rat) 293615
NAD+ ADP-ribosyltransferase activity
protein binding
zinc ion binding
transferase activity
transferase activity, transferring acyl groups other than amino-acyl groups
NAD-dependent histone deacetylase activity
enzyme binding
protein deacetylase activity
NAD-dependent protein deacetylase activity
sequence-specific DNA binding
metal ion binding
NAD+ binding
NAD-dependent protein lysine delactylase activity
protein ADP-ribosylase activity
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylation
aerobic respiration
positive regulation of insulin secretion
cellular response to stress
peptidyl-lysine deacetylation
negative regulation of ERK1 and ERK2 cascade
positive regulation of superoxide dismutase activity
positive regulation of catalase activity
positive regulation of oxidative phosphorylation
positive regulation of ceramide biosynthetic process
negative regulation of reactive oxygen species metabolic process
aging
histone H3 deacetylation
negative regulation of peptidyl-lysine acetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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