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Invitrogen
SIRT3 Polyclonal Antibody
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FIGURE: 1 / 10
SIRT3 Antibody (PA5-96406) in ICC/IF
Immunofluorescence analysis of NAD-dependent protein deacetylase sirtuin-3, mitochondrial was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SIRT3 Polyclonal Antibody (Product # PA5-96406) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secon... View More
Product Details
PA5-96406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: SW620; Cellular Location: Mitochondrion matrix
Immunogen sequence: PQRFLLHVVD FPMADLLLIL GTSLEVEPFA SLTEAVRSSV PRLLINRDLV GPLAWHPRSR DVAQLGDVVH GVESLVELLG WTEEMRDLVQ RETGKLDGPD K
Target Information
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3
Gene Aliases: 2310003L23Rik; AI848213; SIR2L3; SIRT3
UniProt ID: (Human) Q9NTG7, (Mouse) Q8R104
Entrez Gene ID: (Human) 23410, (Mouse) 64384, (Rat) 293615
NAD+ ADP-ribosyltransferase activity
protein binding
zinc ion binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
NAD+ binding
histone deacetylase activity
protein-N-terminal asparagine amidohydrolase activity
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
iprodione amidohydrolase activity
(3,5-dichlorophenylurea)acetate amidohydrolase activity
enzyme binding
4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity
didemethylisoproturon amidohydrolase activity
N-isopropylacetanilide amidohydrolase activity
N-cyclohexylformamide amidohydrolase activity
isonicotinic acid hydrazide hydrolase activity
cis-aconitamide amidase activity
gamma-N-formylaminovinylacetate hydrolase activity
N2-acetyl-L-lysine deacetylase activity
O-succinylbenzoate synthase activity
indoleacetamide hydrolase activity
N-acetylcitrulline deacetylase activity
metal ion binding
N-acetylgalactosamine-6-phosphate deacetylase activity
NAD binding
diacetylchitobiose deacetylase activity
chitooligosaccharide deacetylase activity
protein ADP-ribosylation
protein deacetylation
mitochondrion organization
aerobic respiration
peptidyl-lysine deacetylation
histone H3 deacetylation
negative regulation of peptidyl-lysine acetylation
positive regulation of insulin secretion
negative regulation of ERK1 and ERK2 cascade
negative regulation of reactive oxygen species metabolic process
aging
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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