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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
Assay-dependent | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
SIRT5 Fusion Protein Ag7195 (1-310 aa encoded by BC000126) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.27 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MRPLQIVPS RLISQLYCGL KPPASTRNQI CLKMARPSSS MADFRKFFAK AKHIVIISGA GVSAESGVPT FRGAGGYWRK WQAQDLATPL AFAHNPSRVW EFYHYRREVM GSKEPNAGHR AIAECETRLG KQGRRVVVIT QNIDELHRKA GTKNLLEIHG SLFKTRCTSC GVVAENYKSP ICPALSGKGA PEPGTQDASI PVEKLPRCEE AGCGGLLRPH VVWFGENLDP AILEEVDREL AHCDLCLVVG TSSVVYPAAM FAPQVAARGV PVAEFNTETT PATNRFRFHF QGPCGTTLPE ALACHENETV S (1-310 aa encoded by BC000126)
SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ36950; NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03160}; Regulatory protein SIR2 homolog 5; regulatory protein SIR2 homolog 5 {ECO:0000255|HAMAP-Rule:MF_03160}; silent mating type information regulation 2, S.cerevisiae, homolog 5; sir2-like 5; SIR2-like protein 5; SIR2-like protein 5 {ECO:0000255|HAMAP-Rule:MF_03160}; SIRT5; sirtuin type 5
Gene Aliases: 0610012J09Rik; 1500032M05Rik; AV001953; SIR2L5; SIRT5
UniProt ID: (Human) Q5T294, (Mouse) Q8K2C6, (Rat) Q68FX9
Entrez Gene ID: (Human) 23408, (Mouse) 68346, (Rat) 306840
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