Proteintech

SIRT5 Polyclonal Antibody

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SIRT5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15122-1-AP (SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SIRT5 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SIRT5 Antibody in Immunoprecipitation (IP) — IP result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.

Product Details

15122-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

Assay-dependent

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SIRT5 Fusion Protein Ag7195 (1-310 aa encoded by BC000126)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MRPLQIVPS RLISQLYCGL KPPASTRNQI CLKMARPSSS MADFRKFFAK AKHIVIISGA GVSAESGVPT FRGAGGYWRK WQAQDLATPL AFAHNPSRVW EFYHYRREVM GSKEPNAGHR AIAECETRLG KQGRRVVVIT QNIDELHRKA GTKNLLEIHG SLFKTRCTSC GVVAENYKSP ICPALSGKGA PEPGTQDASI PVEKLPRCEE AGCGGLLRPH VVWFGENLDP AILEEVDREL AHCDLCLVVG TSSVVYPAAM FAPQVAARGV PVAEFNTETT PATNRFRFHF QGPCGTTLPE ALACHENETV S (1-310 aa encoded by BC000126)

Target Information

SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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