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Invitrogen
SIRT5 Polyclonal Antibody
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FIGURE: 1 / 2
SIRT5 Antibody (PA5-13224) in IHC (P)
Immunohistochemistry analysis of SIRT5 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with SIRT5 polyclonal antibody (Product # PA5-13224) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepa... View More
Product Details
PA5-13224
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human SIRT5
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Amount
600 µg
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ36950; NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; Regulatory protein SIR2 homolog 5; silent mating type information regulation 2, S.cerevisiae, homolog 5; sir2-like 5; SIR2-like protein 5; sirtuin type 5; unnamed protein product; yeast silent information regulator 2 (Sir2p) homolog; similar to prokaryotic sirtuins
Gene Aliases: SIR2L5; SIRT5
UniProt ID: (Human) Q9NXA8
Entrez Gene ID: (Human) 23408
NAD+ ADP-ribosyltransferase activity
zinc ion binding
transferase activity
transferase activity, transferring acyl groups other than amino-acyl groups
NAD-dependent histone deacetylase activity
NAD-dependent protein deacetylase activity
protein-malonyllysine demalonylase activity
protein-succinyllysine desuccinylase activity
metal ion binding
protein-glutaryllysine deglutarylase activity
NAD+ binding
protein ADP-ribosylase activity
response to ischemia
protein deacetylation
mitochondrion organization
regulation of ketone biosynthetic process
negative regulation of cardiac muscle cell apoptotic process
response to nutrient levels
protein demalonylation
peptidyl-lysine demalonylation
protein desuccinylation
peptidyl-lysine desuccinylation
protein deglutarylation
negative regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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