Invitrogen

SIRT6 Polyclonal Antibody

View all (18) SIRT6 antibodies

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SIRT6 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of SIRT6 in human mammary cancer tissue. Samples were incubated with SIRT6 polyclonal antibody (Product # PA5-95240).

SIRT6 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of SIRT6 in mouse cardiac muscle tissue. Samples were incubated with SIRT6 polyclonal antibody (Product # PA5-95240).

SIRT6 Antibody in Western Blot (WB) — Western blot analysis of SIRT6 in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: A549 whole cell lysate (40 µg), Lane 3: human placenta tissue lysate (50 µg). Samples were incubated with SIRT6 polyclonal antibody (Product # PA5-95240).

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human SIRT6 recombinant protein (Position: D14-E180).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 5mg BSA, 0.9mg NaCl

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3.5.1.-; NAD-dependent deacetylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; sir2-related protein type 6; SIR2L6; SIRT6; sirtuin type 6

Gene Aliases: 2810449N18Rik; AI043036; SIR2L6; SIRT6

UniProt ID: (Human) Q8N6T7, (Mouse) P59941

Entrez Gene ID: (Human) 51548, (Mouse) 50721

Molecular Function: DNA binding protein chromatin/chromatin-binding protein deacetylase hydrolase nucleic acid binding

post-embryonic cardiac muscle cell growth involved in heart morphogenesis base-excision repair protein ADP-ribosylation negative regulation of cell proliferation regulation of double-strand break repair via homologous recombination protein destabilization response to nutrient levels glucose homeostasis negative regulation of glycolytic process negative regulation of transcription, DNA-templated negative regulation of glucose import positive regulation of fibroblast proliferation histone H3-K9 modification histone H3-K9 deacetylation positive regulation of stem cell proliferation

Process(es)

chromatin binding transcription corepressor activity NAD+ ADP-ribosyltransferase activity NAD(P)+-protein-arginine ADP-ribosyltransferase activity protein binding zinc ion binding NAD-dependent histone deacetylase activity NAD-dependent protein deacetylase activity NAD-dependent histone deacetylase activity (H3-K9 specific) NAD+ binding histone deacetylase activity protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity hydrolase activity iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity protein deacetylase activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity metal ion binding N-acetylgalactosamine-6-phosphate deacetylase activity diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity